国家果树种质南京桃资源圃初级核心种质构建  

Establishment of Peach Primary Core Collection Based on Accessions Conserved in National Fruit Germplasm Repository of Nanjing

作　　者：沈志军[1] 马瑞娟[1] 俞明亮[1] 蔡志翔[1] 许建兰[1] 

机构地区：[1]江苏省农业科学院园艺研究所,南京210014

出　　处：《园艺学报》2013年第1期125-134,共10页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30871681);现代农业产业技术体系建设专项资金资助(CARS-31);农业部种质保护项目(NB2011-2130135-07);国家科技基础条件平台项目(2005DKA21002-21)

摘　　要：利用国家果树种质南京桃资源圃中保存的 630 份资源的 60 个描述项信息,通过比较分组、逐步聚类和随机 3 类取样方法,以及取样比例的筛选,进行了初级核心种质构建研究。结果显示:逐步聚类取样方法优于分组取样和完全随机取样方法;逐步聚类取样方法的最适取样比例为 15%;不同聚类模型间存在差异,聚类后的取样方法间也存在差异;欧式距离和内平方距离法逐步聚类后优先选取高均偏种质的方法更适宜桃初级核心种质的构建。通过缺失表型添加,构建的初级核心种质包括 99 份资源,占原始库的 15.7%,具 100%表型保留比例。经多样性指数、表型方差、变异系数检验,以及数量性状的均值和极差符合率检验,初级核心种质具有良好的代表性。Peach accessions conserved in National Fruit Germplasm Repository of Nanjing were used for establishing primary core collection. Three sampling strategies,classification,stepwise cluster and random,were compared using 630 accessions and 60 descriptors. The sampling scale was also tested with considering the sampling strategies. Stepwise cluster was found to be more suitable for peach than sampled by classification or random. By multiple comparisons,15% sampling ratio was shown to be the best after stepwise cluster. Differences were found among sixteen cluster models and among sampling methods after cluster. Clustered by Euclidean-Ward model and selecting accession with higher value of average degree of deviation was chosen as the best method. A primary core collection of 99 accessions with sampling ratio of 15.7% was established based on the optimal method and by adding absent phenotypes to obtain 100% ratio of phenotype retained（RPR）. The core collection was detected to be well represent the primal collection using Shannon-Wavear index（SI）,variance of phenotype value（VPV）,coefficient of variance（CV）,as well as,detected by coincidence of mean and range for quantity traits.

关 键 词：桃 表型 初级核心种质 

分 类 号：S662.1[农业科学—果树学]