抗菌肽RSRP与常用抗菌药的体外联合药敏试验  

Testing the antibacterial activity of antibacterial peptides in rabbit sacculus rotundus combined with eleven kinds of antibiotics in vitro

作　　者：陈红伟[1,2] 吴俊伟[2] 刘娟[2] 张志强[2] 崔龙萍[2] 李英伦[1] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014 [2]西南大学荣昌校区动物医学系,重庆402460

出　　处：《中国兽医科学》2013年第1期91-94,共4页Chinese Veterinary Science

基　　金：中央高校基本科研业务费专项(XDJK2012C099);重庆市科委自然科学基金计划资助项目(cstc2012jjA80013)

摘　　要：为探讨家兔圆小囊抗菌肽(RSRP)的体外抗菌活性以及与抗菌药之间的协同效应关系,采用琼脂糖弥散试验检测RSRP对8株供试细菌的抗菌活性,然后采用棋盘微量稀释法,选取11种常用抗菌药,分别测定其对临床分离的耐药大肠杆菌的最小抑菌浓度,再采用分级抑制浓度指数来定量检测RSRP与抗菌药之间的抗菌作用关系。结果显示,RSRP对8株供试菌均有不同程度的抗菌活性,分级抑制浓度指数从小于0.3到大于5不等。证实该抗菌肽与不同的抗菌药之间协同、相加、无关和拮抗作用关系均存在,其中与β-内酰胺类药物氨苄西林钠、头孢噻呋钠表现明显的协同作用。To investigate the antibacterial activity of antibacterial peptides from rabbit sacculus rotundus（RSRP） and its synergic interaction with antibiotics,the antibacterial activity of RSRP against 8 bacteria was detected by agarose diffusion assay,and then fractional inhibitory concentration indexes（FICs） of eleven kinds of antibiotics and RSRP were measured by the checkerboard microdilution method.Results demonstrated that there were different degree antibacterial activity of RSRP against the eight bacteria,the FIC values ranged from less than 0.3 to more than 5;and there were synergic,additive,indifference and antagonistic interaction between RSRP and the antibiotics.Among them,there was evidently synergic interaction between RSRP and ampicillin sodium,ceftiofur sodium.

关 键 词：圆小囊 抗菌肽 联合药敏试验 分级抑制浓度指数 

分 类 号：S859.797[农业科学—临床兽医学]