药物对青春期前大鼠单侧睾丸扭转复位后健侧睾丸生精功能的影响  

Effect of Drug on Testicular Spermatogenetic Function of the Contralateral Testicular After Unilateral Testicular Torsion in Prepubertal Rats

作　　者：林孝坤 秦乐[1] 谢添纯[2] 赵军锋[1] 陈聪德[1] 张浩川[1] 陈肖鸣[1] 

机构地区：[1]温州医学院附属育英儿童医院小儿外科,325027 [2]温州医学院附属眼视光学院,325027

出　　处：《医学研究杂志》2013年第1期104-106,共3页Journal of Medical Research

基　　金：国家人口和计划生育委员会基金资助项目(C1-81);温州市科技局基金资助项目(Y20100305)

摘　　要：目的探讨药物对青春期前大鼠单侧睾丸扭转复位后健侧睾丸在青春期后的组织学以及附睾精子生成的影响。方法将28只健康雄性SD大鼠,随机分为假手术组(A组,n=7)、睾丸扭转复位组(B组,n=7)、N-甲基-L-精氨酸(L-NMMA)组(C组,n=7)和环孢素组(D组,n=7)。按Turner法建立左侧睾丸扭转模型,并做相应的药物处理,喂养至术后8周时处死,取右侧睾丸标本行组织病理学观察,同时取右侧附睾尾部行精子计数及活动率测定。结果与B组相比,C组和D组生精上皮层数增多,平均曲细精管直径(MSTD)增大,睾丸组织结构评分降低,同时附睾精子密度与活动率明显升高,其差异有显著性(P<0.05)。结论 L-NMMA和环孢素能够减轻青春期前大鼠单侧睾丸扭转复位后健侧睾丸组织损害,使附睾精子生成显著增多。Objective To investigate the effect of drug on the histological changes and spermatogenesis of the contralateral testis in prepuberty after unilateral testicular torsion in prepubertal rats. Methods Twenty - eight prepubertal male Sprague - Dawley rats were equally randomized into group A （ sham - operation） , group B （torsion/detorsion） , group C （ L - NMMA） and group D （cyclosporine） , with 7 rats in each. The testicular torsion/detorsion model was established by the Turner method,then the rats took the corresponding drug treat- ment. All the rats were fed under the same condition for 8 weeks and sacrificed. Histopathological observation was performed on the right testis. Sperm counting and activity analysis were performed in epididymis. Results Compared with group B, there were significant differences in seminiferous epithelial layers, MSTD and testicular histological score between group C and D. Sperm concentration of epididymis and sperm motility in rats of torsion group were also increased significantly. Conclusion L - NMMA and eyelosporine can reduce the damages of contralateral testis after unilateral testicular torsion in prepubertal rats and increase the spermatogenesis remarkably.

关 键 词：睾丸扭转 L-NMMA 环孢素 生精功能 

分 类 号：R979.5[医药卫生—药品]