荧光定量PCR检测生殖道感染患者HPV6/11型结果分析  

Result Analysis of Fluorescent Quantitative PCR Detection of HPV6/11 Type in Patients with Genitourinary Tract Infection

作　　者：罗慧琴[1] 王志刚[2] 李玲[1] 刘付芹[1] 

机构地区：[1]解放军第264医院检验科,太原030001 [2]海军出版社医疗所,天津300450

出　　处：《临床误诊误治》2013年第1期81-83,共3页Clinical Misdiagnosis & Mistherapy

摘　　要：目的了解生殖道感染人乳头状瘤病毒(HPV)6/11的感染情况,并分析与感染相关的影响因素。方法采用实时荧光定量聚合酶链反应(RT-PCR)技术对我院2010年3月—2012年6月妇科和泌尿外科门诊以生殖道感染症状就诊的725例的生殖道分泌物进行HPV6/11 DNA检测。结果 725例中HPV6/11阳性146例,阳性率20.14%。男性阳性率11.36%(40/352),女性28.43%(106/373),组间比较差异有统计学意义(P<0.01);男、女性≤24岁、25～34岁、35～44岁年龄组阳性率组间比较差异均有统计学意义(P<0.05)。男性HPV6/11 DNA平均定量(4.62±2.13)copies/ml,女性(5.22±2.01)copies/ml。男性各年龄组HPV6/11 DNA平均定量比较差异无统计学意义(P>0.05),女性各年龄组差异有统计学意义(P<0.05)。女性HPV6/11感染以≤34岁高发且病毒含量最高,男性感染则主要集中在25～34岁。结论泌尿生殖道HPV感染不容忽视(尤其女性),RT-PCR定量检测HPV6/11 DNA可为临床判断感染程度及药物疗效提供依据。Objective To investigate the infection of human papillomavirus HPV 6/11 in genitourinary tract and analyze the influencing factors of infection. Methods A real-time fluorescent quantitation polymerase chain reaction （RT-PCR） technology was used to detect HPV6/11 DNA of genital secretions form 725 patients during March 2010 and June 2012 in the outpatient departments of gynecology and urology. Results In 725 cases, there were positive 146 cases, and the positive rate was 20. 14%. The positive rate of males was 11. 36% （40/352）, positive rate of females was 28.43% （106/373）, and the differences of groups were statistically significant （P 〈0.01 ） ; There were significant differences among groups under the age of 424, 95 -34 and 35 -44 in positive rates of both male and female groups （P 〈 0.03）. The average viruses amount was （4.62 ± 2. 13） copies/ml in male and （5.22 ±2.01 ） copies/ml in female. There was no significant difference in average viruses of different age groups in males （P 〉 0. 05 ）, but there were significant differences in female patients （P 〈 0. 05 ）. Infection in most female patients occurred in those under the age of 34 with the highest virus amount, while in most made infection patients occurred under the age of 25 to 34. Conclusion Genitourinary HPV infection should not be ignored （especially in females）. PT-PCR quantitative detection of HPV6/11 DNA may provide valuable information for clinical judgement of infection and curative effect of drugs.

关 键 词：荧光定量 聚合酶链反应 乳头状瘤病毒 人 生殖器官 感染 

分 类 号：R711.3[医药卫生—妇产科学]