天然蒙脱石/植酸酶复合载体制备及其植酸磷水解活性研究  

Preparation of Phytase/Natural Montmorillonite Composite and Its Phytate HydrolysisActivity

作　　者：张闻中[1] 徐芳[1] 徐建雄[2] 

机构地区：[1]上海交通大学环境科学与工程学院,上海200240 [2]上海交通大学农业与生物学院,上海200240

出　　处：《非金属矿》2013年第1期61-63,共3页Non-Metallic Mines

基　　金：科技部中国-斯洛伐克国际合作项目(5-8);国家海洋公益性项目(201005026);上海市科委科学仪器项目(12142200500)

摘　　要：通过吸附过程制备了天然蒙脱石／植酸酶复合载体并系统探讨其催化水解植酸磷活性。结果表明，天然蒙脱石固载植酸酶符合Elovich动力学模型，固载等温曲线满足Langmuir方程，植酸酶最大固载容量为1．48mg／mg；天然蒙脱石／植酸酶复合载体作为微型固定化酶反应器催化水解植酸磷，同自由植酸酶相比较，温度耐受性、抗蛋白酶水解能力均有效改善；适宜的pH值适用范围为4．0-4．5；重复利用5次后酶活保留40％以上。天然蒙脱石／植酸酶复合载体作为饲料添加剂可有效减少植酸酶制剂投加量，提高植酸磷有效利用率，溯源性降低畜禽养殖业“磷”污染排放。Phytase/natural montmorillonite composite was prepared by adsorption process and the composite＇s enzymatic activity for phytate hydrolysis was studied. The results showed that： the kinetic for adsorption of phytase onto natural montmorillonite could be best described by Elovich model, and the adsorptive isotherms fit for Langmuir model, with the maximum monolayer adsorption capacity of 1.48 mg/mg. The phytase/natural montmorillonite composites served as micro enzyme immobilized reactor for phytate hydrolysis. In comparison to free phytasc, the relevant properties of the composites were effectively enhanced in view of the temperature tolerance and anti-proteolytic capability. The suitable pH media range for the composites in real practice was from 4.0 to 4.5. The enzymatic activity retained more than 40% after five testing cycles. Overall, the development of phytasc/natural montmorillonite composite as feed additives could improve the availability of phosphorus from phytate and reduce the dosage of phytase, which contribute to the reduction of phosphorus emissions from livestock and poultry waste.

关 键 词：蒙脱石 固载 植酸酶 活性 

分 类 号：TB34[一般工业技术—材料科学与工程] TD985[矿业工程—选矿]