白芨多糖提取方法的优选及其理化性质研究  

Extracting method optimization and physical and chemical properties of polysaccharide in Bletilla striata

作　　者：韩丹[1] 王艳萍[2] 毕亚静[1] 刘福强[2] 

机构地区：[1]延边大学药学院,吉林延吉133000 [2]解放军208医院,吉林长春130062

出　　处：《药学实践杂志》2013年第1期35-37,共3页Journal of Pharmaceutical Practice

基　　金：国家自然科学基金(81173559)

摘　　要：目的从白芨中等粉中用不同方法提取白芨多糖,优选最佳的提取工艺,对其理化性质进行研究。方法采用水提法、碱水提法、纤维素酶解法、超声波法进行提取;采用分光光度法测定多糖含量。得出多糖得率最高的一种方法为基础,以多糖含量为指标进行工艺优化;按照《中国药典》2010年版一部规定的方法研究其理化性质。结果超声提取法最好,最佳提取工艺为:料液比1∶20,温度80℃,时间10 min,提取率为49.90%;求出5个理化性质数据。结论超声波提取法提取白芨多糖的得率高于其他方法,适宜大规模生产;为白芨多糖现代化应用提供了依据。Objective To establishing the best extraction technology of polysaccharide in Bletilla striata and researching its physical and chemical properties. Methods Water extraction and buck formulation, cellulose enzyme hydrolysis and ultrasonic extrac- tion were used, and the spectrophotometry was used to determine the content of polysaccharide, the receiving rate of polysaccharide in the highest method as the foundation, the contents of polysaccharide as the target to process optimization. The physical and chemical characteristics of polysaccharide were studied according to the Ch. P（2010）. Results The uhrasonie extraction method was the best extraction technologywith the condition that： the ratio of material and liquid was 1 ： 20, temperature was 80 ℃ , in 10 rain. The extrac- tion rate was 49.90%. Five physical and chemical properties were calculated. Conclusion The extraction rate of ultrasonic method for polysaccharide in Bletilla striata was high than other methods, and suitable for mass productionwhich could provide the references for modern application of polysaccharide in Bletilla striata.

关 键 词：白芨多糖 提取 苯酚-硫酸法 工艺优化 理化性质 

分 类 号：R284[医药卫生—中药学]