HPLC法测定盐酸多沙普仑葡萄糖注射液的含量  

Determination of doxapram hydrochloride glucose injection by HPLC

作　　者：薛志红[1] 王盛豪[1] 潘世茹[1] 

机构地区：[1]安徽新华学院药学院,安徽合肥230088

出　　处：《药学实践杂志》2013年第1期64-66,共3页Journal of Pharmaceutical Practice

基　　金：安徽省高等学校省级教学质量与教学改革工程项目(20101142)

摘　　要：目的建立盐酸多沙普仑葡萄糖注射液的高效液相色谱含量测定方法。方法采用COSMOSIL C18(150 mm×4.6 mm,5μm)色谱柱,以0.82μg/L醋酸钠溶液(用冰醋酸调pH值为4.5)-乙腈=70∶30为流动相,检测波长为214 nm。结果盐酸多沙普仑浓度在13～91μg/ml范围内呈良好的线性关系(r=0.999 8)。平均加样回收率为99.3%,RSD为0.81%(n=9)。结论此方法简便、准确、灵敏度高、专属性强。Objective To establish an HPLC method for determination of doxapram hydrochloride glucose injection. Methods COSMOSIL C18 （150 mm× 4.6 mm ,5 μm） column was adopted. The mobile phase consisted of 0.82 p,g/L CH3COONa（ using Gla- cial acetic acid adjust to pH 4.5 ）-acetonitrile （70：30）. The detection wavelength was 214 nm. Results The linearity of doxapram hydrochloride concentration was in the range of 13 - 91μg/ml（ r = 0. 999 8 ）. The average recovery was 99.3 % , RSD = 0.81% （ n = 9）. Conclusion The method was simpleaccurate and sensitive with high specificity.

关 键 词：高效液相色谱法 盐酸多沙普仑 含量测定 

分 类 号：R927[医药卫生—药学]