黄精水煎剂对β-淀粉样蛋白诱导的大鼠学习记忆能力下降的保护作用研究  

Protective effects of Polygonatum cyrtonema decoction on beta-amyloid peptide-induced learning and memory decline in rats

作　　者：王涛涛[1] 程娟[1] 姚余有[1] 

机构地区：[1]安徽医科大学公共卫生学院,合肥230032

出　　处：《安徽农业大学学报》2013年第1期95-99,共5页Journal of Anhui Agricultural University

基　　金：安徽高校省级自然科学研究项目(KJ2011Z161);安徽省自然科学基金(1208085MH145)共同资助

摘　　要：本研究旨在探讨黄精水煎剂(PCD)对β-淀粉样蛋白(Aβ)致阿尔茨海默病(AD)模型大鼠的保护作用及可能机制。通过Aβ25-35微量注射至大鼠双侧海马CA1区建立AD模型,同时用PCD干预,研究PCD对AD模型大鼠学习记忆能力、海马组织损伤和CA1区神经元中过度磷酸化tau蛋白(p-tau)表达的影响。结果表明,PCD可明显缩短模型大鼠逃避潜伏期,对模型大鼠学习记忆能力损伤有保护作用;能改善大鼠海马神经元损伤,减少CA1区在thr231位点tau蛋白的过度磷酸化。这些结果提示,PCD对学习记忆能力有显著的保护作用,这可能与其减少海马内p-tau-thr231水平和消除组织氧化逆境有关。In order to investigate the protective effects and underlying mechanisms of Polygonatum cyr- tonema decoction （PCD） on amyloid β-peptide （Aβ）-induced Alzheimer＇s disease （AD） rats, a AD model was es- tablished by injecting Aβ5-35 into the CA1 field of hippocampus, and PCD was simultaneously administered in- tragastrically to the rats. The learning and memory ability, the histopathologic changes and the expression of phospho-tau protein in CA1 field of hippocampus were evaluated in all the rats. The results showed that PCD ob- viously shorten escape latency of model rats, improve histopathologic changes and reduce the excessive expres- sion ofp-tau protein on the site of thr231 in CA1 field of hippocampus in model rats. These results suggested that PCD has protective effects on Aβ25-35-induced learning and memory decline in rats, which is likely to be related with its function of reducing the excessive expression ofp-tau protein on the site of thr231 and eliminating oxida- tive stress in tissue.

关 键 词：黄精 阿尔茨海默病 学习/记忆 p-tau-thr231 

分 类 号：R338.64[医药卫生—人体生理学] S865.12[医药卫生—基础医学]