白芍总苷对非酒精性脂肪性肝病大鼠Apelin和Visfatin表达的影响  

Effect of Total Glucosides of Paeony on Apelin and Visfatin Expression in Rats with Nonalcoholic Fatty Liver Disease

作　　者：郑琳颖[1] 潘竞锵[1] 杨以琳[1] 吕俊华[2] 赵汝霞[2] 肖瑛[2] 周永标[3] 潘卫松[3] 

机构地区：[1]广州市中医医院,广东广州510130 [2]暨南大学药学院,广东广州510632 [3]广州市药品检验所,广东广州510160

出　　处：《中药新药与临床药理》2013年第1期51-54,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广州市科技计划项目(2010Y1-C491);广州市中医药中西医结合科技项目(2009-A-10)

摘　　要：目的观察白芍总苷(TGP)对非酒精性脂肪性肝病(NAFLD)大鼠及脂肪因子apelin和visfatin的影响。方法以果糖-高脂诱导非酒精性脂肪性肝病大鼠模型,模型大鼠随机分为模型组,二甲双胍组(200 mg.kg-)1,TGP高、低剂量组(200,100 mg.kg-)1,另设正常对照组。用药4周后观察各组大鼠空腹血糖(FBG)、胰岛素(Fins)、胰岛素敏感指数(ISI)、胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、甘油三酯(TG)、游离脂肪酸(FFA)、apelin-36、visfatin、天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、谷胱甘肽转移酶(GST)和肝脏指数。结果 TGP高、低剂量组FBG、Fins、LDL-C、TG、apelin-36和visfatin均明显降低,ISI和HDL-C含量明显升高(P<0.01或P<0.05),TGP高剂量组能显著降低ALT、AST和GST的活性及肝脏指数(P<0.01),TGP低剂量也能显著降低A ST和GST的活性(P<0.01)及肝脏指数(P<0.05)。结论 TGP能改善果糖-高脂诱导NAFLD大鼠糖脂代谢异常及拮抗胰岛素抵抗,增强胰岛素敏感性,下调apelin和visfatin的表达,改善肝功能。Objective To investigate total glucosides of paeongy（TGP） on apelin and visfatin in rats with nonal- coholic fatty liver disease（NAFLD）. Methods SD rats were divided into normal group, model group（NAFLD）, metformin group（MET, 200 mg·kg-1） , high-dose TGP group（TGP-H, 200 mg·kg-1） and low-dose TGP group （TGP-L, 100 mg·kg-1）. NAFLD rat model was induced by fructose and high-fat feeding. After treatment for 4 weeks, all the rats were executed for the examination of serum fasting blood glucose（FBG）, fasting insulin （Fins）, insulin sensitivity index（ISI）, total cholesterol（TC）, low-density lipoprotein cholesterol（LDL-C）, high- density lipoprotein cholesterol （HDL-C）, triglyceride （TG）, free fatty acids （FFA）, apelin-36, visfatin, alanine aminotransferase（ALT）, aspartate aminotransferase（AST）, glutathione transferase （GST） levels and liver index.Results In both TGP-H group and TGP-L group, the levels of FBG, Fins, TG, LDL-C, apelin-36 and vis- fatin were decreased, but ISI and HDL-C were increased（P 〈 0.01 or P 〈 0.05）. The activities of ALT, AST, and GST as well as the liver index were reduced by TGP-H （P 〈 0.01）. The AST and GST activities and the liver index were also reduced by TGP-L（P 〈 0.01 or P 〈 0.05）. Conclusion TGP could improve the disordered lipid metabolism and insulin resistance, enhance insulin sensitivity, down-regulate apelin and visfatin expression, and ameliorate liver function in rats with NAFLD induced by fructose and high-fat feeding.

关 键 词：白芍总苷 非酒精性脂肪性肝病 胰岛素抵抗 二甲双胍 APELIN VISFATIN 

分 类 号：R285.5[医药卫生—中药学]