姜辛夏颗粒对哮喘大鼠气道重建中胶原蛋白及支气管壁厚度的影响  

Effect of Jiang Xin Xia Granules on Collagen Expression and Bronchial Wall Thickness in Asthmatic Rats with Airway Remodeling

作　　者：张玉英[1] 杨军[1] 王礼凤[1] 何云义[1] 惠朋利[1] 应小平[1] 

机构地区：[1]陕西中医学院,陕西咸阳712046

出　　处：《中药新药与临床药理》2013年第1期63-65,107,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：陕西省教育厅专项计划项目(08JK0275);陕西省教育厅重点学科(伤寒论)资助项目

摘　　要：目的研究姜辛夏颗粒对卵蛋白(OVA)致敏哮喘大鼠模型肺组织中Ⅰ、Ⅲ、Ⅴ型胶原蛋白表达及对支气管壁厚度的影响。方法采用OVA致敏建立哮喘大鼠模型,观察姜辛夏颗粒对哮喘大鼠的疗效;免疫组化技术检测肺组织中Ⅰ、Ⅲ、Ⅴ型胶原蛋白的表达;支气管组织HE染色,计算机图像分析测定气道壁厚度。结果与模型组比较,姜辛夏颗粒能明显减轻哮喘大鼠的哮喘症状和支气管黏膜的炎症反应;明显缩小哮喘大鼠支气管总管壁面积(WAt)、内壁面积(WAi)及平滑肌面积(WAm)(P<0.01);明显降低肺组织中I、Ⅲ、V型胶原蛋白的表达(P<0.01,P<0.05)。结论姜辛夏颗粒可明显降低哮喘大鼠支气管上皮下Ⅰ、Ⅲ、Ⅴ型胶原蛋白的合成和沉积,减小支气管壁的厚度,改善支气管的通气功能,从而抑制哮喘气道重建的发生发展。Objective To study the effect of Chinese herbal medicine Jiang Xin Xia granules on the expression of collagen type Ⅰ、Ⅲ and Ⅴ in the lung tissue and bronchial wall thickness of ovalbumin （ OVA ）-induced asthmatic rat model. Methods OVA-induced asthmatic rat model was replicated and the medication groups were given intragastric administration of the corresponding drugs respectively. After treatment, therapeutic effect was evaluated in all of the groups. The expression of collagen type Ⅰ、Ⅲ and Ⅴ in the pulmonary tissues was detected by using immunohistoehem- istry technique, and the bronchial wall thickness was measured after HE staining through computer imaging analysis. Results Compared with the asthmatic model group, Jiang Xin Xia granules had an obvious effect on relieving asthma and bronchial mucosal inflammatory reaction in asthmatic rats, reducing the bronchial total wall area, inner wall area and smooth muscle area （P 〈 0.01 ）,and decreasing the expression of collagen type Ⅰ、Ⅲ and Ⅴ in the pulmonary tissues（P 〈 0.01 or P 〈 0.05）. Conclusion Jiang Xin Xia granules can obviously decrease the synthesis and deposition of collagen Ⅰ、Ⅲ and Ⅴ under bronchial epithelia, reduce airway wall thickness, and improve bronchial ventilation function, thus to prevent the occurrence and development of asthmatic airway remodeling.

关 键 词：支气管哮喘 姜辛夏颗粒 胶原蛋白 支气管壁厚度 

分 类 号：R285.5[医药卫生—中药学]