藻黄合剂含药血清对高糖环境下人肾小球系膜细胞MCP-1表达的影响  

Effects of serum containing Zaohuang Mixture on the expression of monocyte chemotactic protein-1 in human mesangial cells cultured with high glucose

作　　者：鞠建伟[1] 贺玉珍[2] 张李梅[2] 尹燕志[1] 牟授菡[1] 吕丛奎[1] 

机构地区：[1]烟台经济技术开发区医院,烟台264006 [2]滨州医学院,烟台264003

出　　处：《中华中医药杂志》2013年第2期406-409,共4页China Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:观察藻黄合剂(ZHM)对高糖环境下人肾小球系膜细胞(HMC)单核细胞趋化蛋白1(MCP-1)表达的影响,探讨其在糖尿病肾脏疾病(DKD)防治中的意义。方法:体外培养HMC并鉴定,血清药理学方法制备ZHM低、中、高剂量组,空白对照组及海昆肾喜阳性对照组大鼠含药血清,10%各组含药血清培养基分别孵育高糖作用下HMC 48h,另设低糖组作为空白对照。ELISA法和RT-PCR技术分别检测各组系膜细胞MCP-1蛋白及mRNA表达情况。结果:MCP-1蛋白及mRNA表达量在高糖组较低糖组明显升高(P<0.01);ZHM中、高剂量组MCP-1蛋白及mRNA表达量均低于高糖组,且具有剂量依赖性;ZHM中、高剂量组与海昆肾喜组比MCP-1蛋白及mRNA表达差异无统计学意义。结论:高糖可诱导HMC中MCP-1表达,而ZHM可通过抑制高糖条件下HMC中MCP-1蛋白及mRNA表达,发挥对DKD的防治作用。Objective： By investigating the effect of Zaohuang Mixture （ZHM） on the expression of monocyte chemotactic protein-1 （MCP-1） in human mesangial cells （HMC） which cultured under high glucose condition, to explore the significance of kidney protection in diabetic kidney disease（DKD）. Methods： HMC were cultured in vitro and identified. Serums containing different dose （low, medium, high dose） of ZHM and Haiknn Shenxi were prepared by serum pharmacology method. HMC were incubated in DMEM medium with 10% contained serum and high glucose for 48 hours. And low glucose as the control group. The level of MCP-1 protein and mRNA expression was measured by ELISA and Reverse Transcriptase Polymerase Chain Reaction （RT-PCR）, respectively. Results： With the stimulation of high glucose, the expression of MCP-1 protein and mRNA was increased significantly （P〈0.01）, which then were decreased by the treatment of ZHM and showed character of dose dependent. Compared with Haikun Shenxi group, MCP-1 protein and mRNA expression in ZHM high-dosage group and middle-dose group was not statistically significant. Conclusion： The expression of MCP-I in HMC might be induced by high glucose and ZHM could inhibit the expression for prevention and treatment of DKD.

关 键 词：藻黄合剂 人肾小球系膜细胞 血清药理学 高糖 单核细胞趋化蛋白1 

分 类 号：R285.5[医药卫生—中药学]