HPLC法测定不同季节千层塔中石杉碱甲和石杉碱乙含量  

Determination of Huperzine A and Huperzine B in Huperzia serrata collected at diffefent collecting times by HPLC

作　　者：周小雷[1] 袁经权[1] 王硕[1] 闫志刚[1] 冯世鑫[1] 蒋山好[2] 朱大元[2] 马小军[1] 

机构地区：[1]广西药用植物研究所,南宁530023 [2]中国科学院上海药物研究所,上海201203

出　　处：《中华中医药杂志》2013年第2期504-506,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广西科技攻关项目(No.桂科攻11107010-2-4);广西区科技厅应用基础研究专项(No.桂科基0991019);广西区科技厅青年基金项目(No.2010GXNSFB013041);广西南宁市科技攻关项目(No.201102076C);广西区卫生厅重点项目(No.重2011031);广西科技创新能力与条件建设(No.桂科能10100027-3)~~

摘　　要：目的:考察不同季节千层塔药材中石杉碱甲、乙的含量,为确定千层塔的最佳采收期提供依据。方法:采用超声提取方法进行样品的制备;用HPLC方法测定千层塔中石衫碱甲、石衫碱乙的含量。结果:石杉碱甲、乙线性范围分别为0.050μg-1.000μg、0.031μg-0.620μg,回收率分别为100.1%(RSD:0.72%,n=6)、99.5%(RSD:1.1%,n=6)。不同采收期千层塔中的石衫碱甲、石衫碱乙的含量差异较大,其中8月份的含量最高,为0.0236%、0.0124%。结论:该研究可为千层塔最佳采收期的确定提供参考,以8月份为千层塔药材的最佳采收时期。Objective： To study the contents of Huperzine A and Huperzine B from Huperzia serrata collected at different collecting times by HPLC to decide the optimal harvest time. Method： Ultrasonic extraction method for sample preparation was performed, the determination of Huperzine A in Huperzia serrata by HPLC. Results： The linear range of Huperzine A was 0.050μg-1.000μg, and Huperzine B was 0.031μg-0.620μg, the average recovery were 100.1% （RSD：O.72%, n=6 ） and 99.5% （RSD：1.1%, n=6 ） , collected at different collecting times the contents of Huperzine A and Huperzine B in Huperzia serrata were evidently different, the hightest in August, the contents of Huperzine A was 0.0236% and Huperzine B was 0.0124%. Conclusions： The best collecting time of the plant is August as provided the reference from this study.

关 键 词：千层塔 不同季节 石杉碱甲 石杉碱乙 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]