针刺对神经干细胞海马内移植痴呆大鼠海马组织超微结构的影响  

Effects of acupuncture on the ultrastructure of hippocampus of neural stem cell of Alzheimer's disease rats

作　　者：杨春壮[1] 马英[2] 张凡[1] 徐永良[1] 王莹[1] 李文媛[1] 刘跃光[1] 

机构地区：[1]牡丹江医学院解剖教研室,牡丹江157011 [2]牡丹江医学院图书馆,牡丹江157011

出　　处：《中华中医药杂志》2013年第2期541-543,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：黑龙江省自然科学基金项目(No.D200664);黑龙江省卫生厅医学科研课题(No.2006-401);黑龙江省中医药科研项目(No.ZHY10-W97)~~

摘　　要：目的:观察针刺对神经干细胞(NSCs)海马内移植老年性痴呆(AD)大鼠海马组织超微结构的影响,探讨针刺治疗老年性痴呆的机制。方法:采用β-淀粉样蛋白(β-AP)向海马CA1区定向注射制做AD模型,将大鼠分为5组:正常组、模型组、移植组、针刺组和移植针刺组,每组各12只。针刺选取百会、大椎、人中3个穴位,并接通电针仪。大鼠跳台检测行为学指标;通过电镜观察海马组织超微形态学。结果:移植针刺组大鼠跳台学习、记忆所需次数比模型组减少(P<0.05),且均优于移植组(P<0.05);电镜观察到AD大鼠脑组织海马区神经元均有不同程度的破坏。模型组大鼠海马区神经元细胞质明显水肿,异染色质明显增加;移植针刺组大鼠海马区神经元细胞损伤程度比模型组明显减轻。结论:电针对于AD模型大鼠的神经结构具有明显的保护作用,减少神经元的损伤,且针刺结合神经干细胞移植治疗效果更佳。Objective： To observe the effects of acupuncture on the ultrastructure of hippocampus of Neural stem cell of Alzheimer＇s disease （AD） rats and discuss the mechanism of acupuncture on the AD. Methods： The model was made by injection β-AP to CAt of hippocampus, the rats were randomized into five groups, including normal, model, transplant, acupuncture and transplant acupuncture group, 12 rats of each group. Baihui（DU20）, Dazhui（GV14） and Renzhong（DU26） were selected as acupoints and used electric acupuncture apparatus. The memory ability was measured by use of the diving tower of rats and the ultrastructure of hippocampus were observed by electron microscope. Results： The times of the ability of study and memory of step down in transplant acupuncture group were less than the model group（P〈0.05）, moreover it significantly better than transplant group（P〈0.05）; the neurons of AD rats in brain hippocampus were damaged in varying degrees. The cytoplasms in hippocampus of the model group became obvious edema, the heterochromatin abviously increased; while the damaged degree of the neuron in hippocampus in the transplant acupuncture group was clearly decreased than the model group. Conclusion： The structure of nerve cell in AD rats could be abviously protected by the electric acupuncture, and the damaged neurons were decreased. Electric needle combined with transplant treatment had a better effect than others.

关 键 词：老年性痴呆 神经干细胞 海马 超微结构 

分 类 号：R245[医药卫生—针灸推拿学]