猪天然管状SIS人工食管的制备和不同灭菌方式的比较  

Preparation of natural tubular artificial esophagus from porcine small intestinal submucosa and comparison of different sterile manner

作　　者：李春霖[1] 薛锦儒[1] 吴金凤[2] 崔建华[1] 

机构地区：[1]吉林大学中日联谊医院胸外科,吉林长春130033 [2]长春大学医院,吉林长春130022

出　　处：《中国实验诊断学》2013年第1期15-19,共5页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金资助(项目批准号:31070879)

摘　　要：目的天然管状细胞外基质的制取及灭菌方式的比较。方法以物理方法刮除猪小肠的肌层与粘膜层制取天然管状细胞外基质膜,对比粒子加速器的不同辐照剂量与过氧化氢低温等离子灭菌的效果。结果 25KGy辐照剂量与过氧化氢低温等离子的灭菌方法均可达到理想的灭菌效果。结论粒子加速器25KGy辐照剂量的灭菌方式更适合细胞外基质膜灭菌处理。Objective Decellularized porcine small intestinal submucosa（SIS）, fabricated a natural tubular extracellu- lar matrices and comparison the effect of different sterile manner. Methods Physical methods to remove the porcine small intestine muscle layer and mucosal layer to fabricated of the natural tubular extracellular matrix membrane, the effect of the comparison of particle accelerator with different radiation dose and low temperature hydrogen peroxide plasma sterilization. Results Irradiation dose of 25 KGy and low temperature hydrogen peroxide plasma sterilization all can achieve ideal effect of sterilization. Conclusion The sterilization of particle accelerator with 25KGy irradiation dose is more suitable for extracellular matrix membrane .

关 键 词：细胞外基质 人工食管 组织工程 灭菌方法 

分 类 号：R318.14[医药卫生—生物医学工程] R187[医药卫生—基础医学]