涝胁迫对不同丹参品系苗期保护酶活性及脂质过氧化作用的影响  

Effects of water stress on protective enzyme activities and lipid peroxidation in different Salvia miltiorrhiza Bunge varieties at seedling stage

作　　者：路萍[1] 张利[1] 王萌[1] 王涛[1] 姜媛媛[1] 

机构地区：[1]四川农业大学生命科学与理学院,四川雅安625014

出　　处：《浙江大学学报（农业与生命科学版）》2013年第1期56-61,共6页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家农业科技成果转化资金项目(2012GB2F000385);四川省科技厅科技支撑计划项目(2012NZ0020;2011ZO0006);四川省科技厅软科学计划项目(2012ZR0050)

摘　　要：以四川省中江县6个丹参品系幼苗为试材,采用土培模拟涝害的方法,研究涝胁迫对丹参幼苗叶片超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)、过氧化氢酶(catalase,CAT)活性及游离脯氨酸(proline,Pro)质量分数和丙二醛(malondialdehyde,MDA)质量摩尔浓度的变化。结果表明:涝胁迫5d作为模拟涝胁迫的最佳条件。在受到涝胁迫时,组培大叶丹参的SOD和CAT活性最高,组培小叶丹参的最低;矮秆大叶丹参的POD活性最高,组培小叶丹参的最低;四倍体小叶丹参的Pro质量分数和MDA的质量摩尔浓度最高,中江野生丹参的Pro质量分数最低;组培大叶丹参、高秆大叶丹参和矮秆大叶丹参的MDA质量摩尔浓度相同,且低于其他3个品系。对各项生理生化指标的相关性分析表明,各指标相关性不显著,彼此呈独立作用态势,均可作为鉴定丹参抗涝性的生理指标。Summary The dried root and rhizome of Salvia miltiorrhiza Bunge （Lamiaceae, Salvia）, one of the popular traditional Chinese medicine （TCM）, is officially listed in Chinese pharmacopoeia under the name of Danshen. S. miltiorrhiza have been widely used for treatment of coronary heart disease, cerebrovascular disease, neurasthenic insomnia, bone toss, liver fibrosis, hepatocirrhosis and chronic renal failure. Waterlogging is abiotic stress that influences species composition and productivity in numerous plant communities worldwide. For most crops, excess water is a major constraint to productivity in many regions and situations, and the inability of crops to withstand low oxygen conditions in the root zone will lead to yield losses. Zhongjiang County was the genuine producing areas with a long history for the plant of Danshen. But now, the effect mechanism of water stress on S. rniltiorrhiza was little known. The objective of this study was to elucidate the physiological responses of different S. miltiorrhiza under water stress. The finite element method can be used as academic base for flooding resistance mechanisms, and provide evidence for breeding S. miltiorrhiza varieties and improving the cultivation technology of resistance to waterlogging. The experiment was conducted in 2010--2011 at the experimental station of Sichuan Agricultural University, Ya＇an, Sichuan Province, China. Tissue cultured lobular S. miltiorrhiza, tissue cultured big-leaf S. miltiorrhiza, tetraploid lobular S. miltiorrhiza, tall big-leaf S. miltiorrhiza, dwarf big-leaf S. miltiorrhiza and wild S. miltiorrhiza in Zhongjiang were obtained from Zhongjiang County, Sichuan Province. All materials were maintained in a rain shelter. The waterlogging treatment was mimicked by treating pot plants with flooding. Investigations were carried out on the changes of malondialdehyde （MDA） content, superoxide dismutase （SOD）, peroxidase （POD）, eatalase （CAT） activities and proline （Pro） content of six S. miltiorrhiza varietie

关 键 词：丹参 涝胁迫 超氧化物歧化酶 过氧化物酶 过氧化氢酶 丙二醛 游离脯氨酸 

分 类 号：S567.23[农业科学—中草药栽培] Q945.78[农业科学—作物学]