甘蔗SPS Ⅲ启动子区ATCT-motif和CAT-box元件的酵母单杂交报告载体构建  

Construction of Yeast One-hybrid Reporter Vector for Screening the Binding Proteins of ATCT-motif and CAT-box in SPS Ⅲ Promoter

作　　者：张积森[1,2,3] 林清凡[1,2,3] 方静平 邹丽娟[1,2,3] 叶冰莹[1,2,3] 陈由强[1,2,3] 陈如凯 

机构地区：[1]福建师范大学生命科学学院,福建福州350117 [2]农业部福建甘蔗生物学与遗传育种重点实验室,福建福州350117 [3]发育与神经生物学福建省高等学校重点实验室,福建福州350117

出　　处：《福建师范大学学报（自然科学版）》2013年第1期86-89,100,共5页Journal of Fujian Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(31201260);农业部现代农业产业技术体系建设专项资金(CARS-20-4-4);福建省教育厅资助项目(JA12082)

摘　　要：蔗糖磷酸合成酶是植物蔗糖代谢中关键限速酶之一,为了更具体分析光响应元件ATCT-motif与分生组织特异性元件CAT-box的功能,将含有ATCT-motif和CAT-box的SPSⅢ5'侧翼序列(-1 320～-1 210),顺式作用元件SPSⅢ-AB,构建应用酵母单杂交体系的诱饵报告载体pAbAi-SPSⅢ-AB,并转化进酵母,为进一步探讨SPSⅢ基因的调控序列奠定基础.Sucrose phosphate synthase is the key enzyme for sucrose accumulation in plant, which contains two specific domain in its promoter region, light-response element ATCT-motif and meristem-specificity element CAT-box. To concretely analyze the function of ATCT-motif and CAT- box from sucrose phosphate synthase Ⅲ in sugarcane, the fragment containing these two elements from promoter region （ - 1 320- - 1 210） was cloned and inserted into yeast one-hybrid bait vector to construct pAbAi-SPS Ⅲ-AB vector which then was transformed to yeast to screen the binding proteins. It provided a framework for further investigation on the regulation mechanism of SPS gene in sugarcane.

关 键 词：酵母单杂交 蔗糖磷酸合成酶基因Ⅲ 转录因子 启动子 

分 类 号：Q781[生物学—分子生物学] Q946