WT对辐射致DNA损伤修复的影响  

Wortmannin inhibits repair of DNA double strand breaks induced by radiation.

作　　者：李雨[1] 赵芳[1] 郑秀龙[1] 罗成基[2] 蔡建明[1] 

机构地区：[1]第二军医大学放射医学教研室,上海200433 [2]第三军医大学防原教研室

出　　处：《中华放射医学与防护杂志》2000年第3期176-178,共3页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的　研究磷脂酰肌醇 3 -激酶的特异性抑制剂Wortmannin(WT)对小鼠胸腺细胞DNA辐射损伤修复的影响 ,以探索WT放射增敏作用机制。方法　以 50 μmol/LWT作用小鼠胸腺细胞 ,以不同剂量γ射线照射 ,照射后不同时间制取细胞DNA ,用琼脂糖凝胶电泳方法检测DNA双链断裂 (dsb)。结果　WT单独对受照射细胞dsb产额无明显影响 ,抑制照射后dsb修复。Objective\ To study the effect of the specific inhibitor Wortmannin(WT)on repair of radiation damage of thymocyte DNA in mice. Method The cells were exposed to WT or DMSO(solvent)for 1 h,and then irradiated at the indicated doses.After indicated times post irradiation,the cells were washed to remove the WT,their DNA was made out,then DNA damage and repair were detected by electrophoresis. Results After 10 Gy and 20 Gy γ irradiation,dsb level was higher in WT group than in control group.It was more evident at 1-3 hrs after irradiation. Conclusion The radiosensitizing effect of WT is related to its inhibition of dsb repair after irradiation.\;

关 键 词：WORTMANNIN 放射增敏 DNA双链断裂 DNA损伤 

分 类 号：R14[医药卫生—公共卫生与预防医学]