毒死蜱和乐果联合染毒对大鼠胆碱酯酶活性的影响  

Combined toxic effect of chorpyriphos and dimethoate on cholinesterase in rats

作　　者：吴惠[1] 乙楠楠[1] 李亭亭[1] 何贤松[1] 赵敏娴[1] 姚欣雅[1] 王灿楠[1] 

机构地区：[1]东南大学公共卫生学院环境医学工程教育部重点实验室,东南大学公共卫生学院营养与食品卫生系,江苏南京210009

出　　处：《癌变．畸变．突变》2013年第1期31-34,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金资助项目(81072304)

摘　　要：目的:研究毒死蜱和乐果联合染毒对大鼠血清和脑组织乙酰胆碱酯酶(acetylcholinesterase,AChE)和丁酰胆碱酯酶(butyrylcholinesterase,BuChE)活性的影响。方法:采用3×3析因设计,雌性SD大鼠按体质量随机分为9个剂量组和1个阴性对照组,每组10只。选用毒死蜱和乐果的无可见有害作用水平(no observed adverse effect level,NOAEL)、1/100半数致死剂量(medianlethaldose,LD)和1/50LD作为低、中、高3个剂量水平,分别为毒死蜱0.03、1.35、2.70mg/(kg·d),乐果1.20、3.25、6.505050mg/(kg·d)。灌胃染毒30d,染毒结束后,经乙醚麻醉,股动脉取血,脱臼处死,剥取大脑皮质和海马,使用胆碱酯酶试剂盒测定胆碱酯酶活性。结果:染毒30d后,高剂量组大鼠胆碱酯酶活性低于对照组(P<0.05);析因设计方差分析显示,毒死蜱和乐果联合染毒,对大鼠血清和皮质AChE活性无交互影响;对海马AChE和血清、脑组织BuChE活性存在交互作用(P<0.01)。结论:经口染毒高剂量有机磷农药可显著抑制血清和脑组织的胆碱酯酶活性,两种有机磷农药混配后可对胆碱酯酶活性产生交互作用。OBJECTIVE： To study the combined toxic effect of chorpyriphos and dimethoate on plasma and brain cholinesterase activities in female rats. METHODS： 3 × 3 factorial analysis was used in this experiment. Adult female SD rats were randomly divided into 10 groups of ten rats （including a control group and nine treated groups） according to their weight. The NOAEL （no observed adverse effect level）, 1/100 LD50 （median lethal dose）and 1/50 LD50 of chlorpyrifos and dimethoate were selected as the low, medium, and high dose level, respectively. The dose of chlorpyrifos were 0.03, 1.35, 2.70 mg/ （kg·d） respectively, and those of dimethoate were 1.20, 3.25, 6.50 mg/ （kg·d） respectively. Blood was collected through arteria cruralis after 30 days of treatment via gastric gavage, then rats were killed and tissues including cortex and hippocampus were collected. Cholinesterase activity was estimated by the Pureauto CHE. RESULTS： The difference of ChE activity between high dose group and control group was significant. No interaction was noted between chorpyriphos and dimethoate on plasma and cortex ACHE. The combination of chorpyriphos and dimethoate caused an interactive effect on plasma and brain BuChE inhibition and hippocampus AChE inhibition （P〈 0.01）. CONCLUSION： High dose combination of organophosphorus pesticides could significantly inhibit the activity of ChE in blood and brain, and also lead to an interactive effect on plasma and brain ChE inhibition.

关 键 词：毒死蜱 乐果 乙酰胆碱酯酶 丁酰胆碱酯酶 联合作用 析因分析 

分 类 号：R995[医药卫生—毒理学]