卷丹小孢子母细胞减数分裂染色体行为的研究  

Chromosom Behavior of Pollen Mother Cell in Meiosis Process for Lilium lancifolium

作　　者：刘晓丽[1] 贾桂霞[1] 

机构地区：[1]北京林业大学园林学院/国家花卉工程技术中心,北京100083

出　　处：《植物遗传资源学报》2013年第1期152-158,共7页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(31071819);国家科技支撑计划(2009BADB8B04);北京市教育委员会产学研合作项目(北京地区百合种球和切花产业化生产关键技术推广示范)

摘　　要：对引种于陕西汉中地区的三倍体卷丹的小孢子母细胞减数分裂进程、花粉生活力和可育性进行了分析。结果显示:减数分裂时期与花蕾、花药长度及花药壁颜色呈现出一定的相关性,处于减数分裂时期的卷丹花蕾长度在2.5～3.2 cm之间,花药长度在1.4～1.8 cm之间;从细线期到成熟花粉粒,花药壁的颜色呈现出淡绿色-淡黄绿色-黄绿色-橘黄色的变化趋势,花药内容物颜色也从透明逐渐变为粘稠的橘红色。减数分裂终变期染色体构型以三价体为主,平均构型为:11.6Ⅲ+2Ⅱ+1.1Ⅰ,可判断该种源地卷丹为同源三倍体。减数分裂中出现染色体不均等分裂、染色体落后、染色体桥及微核现象,同时观察到成熟花粉粒大小差异较大,极轴与赤道轴乘积在839.465～3327.907μm2范围内,趋于正态分布,为三倍体卷丹花粉的高度不育提供了细胞学证据。以卷丹为母本与大花卷丹进行杂交,得到一定数量的种子,其中有胚率达到了43.0%,成苗率为67.4%,说明三倍体卷丹在百合育种中具有一定的价值。The meiosis process of pollen mother cell, the pollen grains vitality of Lilium lancifolium from Hanzhong district,Shanxi province,was observed. The results showed that the length of buds and anthers, and the color of the anther wall were related to the stage of the meiosis process. The length of buds and anther which were in the meiosis process were between 2.5 and 3.2 cm/1.4 and 1.8 cm, respectively. The main chromosome configura- tions were trivalent in diakinesis stage, and the average configuration was 11.6 II1 ＋ 2 11 ＋ 1.1 I , which showed that the L. lancifolium was used in this experiment was autotriploid. The asymmetric division, chromosome lag, chromo- some bridge,and micronucleus were present in distinct meiosis stages,which provided the low fertility of pollen grain. The prospect of application of tripolid L. lancifolium in discussed in this article. e cytological evidence for the Lily breeding was also

关 键 词：卷丹 小孢子母细胞 减数分裂 三倍体育性 

分 类 号：S682.29[农业科学—观赏园艺]