流式细胞术对冠心病患者血小板活化状态的检测  

FLOW CYTOMETRIC MEASUREMENT OF PLATELET ACTIVATION IN PATIENTS WITH CORONARY HEART DISEASE

作　　者：张锑[1] 张春青[1] 徐从高[1] 李贵双[2] 张梅[2] 高海青[2] 

机构地区：[1]山东医科大学附属医院血液病研究室 [2]山东医科大学附属医院心内科

出　　处：《山东医科大学学报》2000年第2期177-178,187,共3页Acta Academiae Medicinae Shandong

摘　　要：目的 :观察正常人和冠心病患者体内血小板活化状态并探讨其临床意义。方法 :用流式细胞术 (FCM ) ,以单克隆抗体为探针 ,对 113例冠心病患者及 37例健康人血小板活化标记物CD63、CD62P、凝血酶敏感蛋白 (TSD)进行了检测。结果 :患者组 3种血小板活化标志物阳性表达率均高于正常对照组 (P <0 .0 0 1)。结论 :用FCM作为活化血小板的检测手段 ,结果准确 ,特异性和灵敏度高 ,可反映单个或亚群血小板质膜上活化抗原的变化 。Objective:To evaluate the status of in vivo platelet activation in normal subjects and patients with coronary heart disease(CHD).Methods:Platelet activation markers including membrane glycoproteins of CD 63 ,CD 62P and thrombospondin were fluorescencelabled with their corresponding monoclonal antibodies and analyzed flowcytometrically.Results:The expression of the three glycoproteins measured were significantly stronger in 113 patients with CHD than in the 37 normal subjects.A trend of increasing expression of the glycoproteins was observed in relation to the severity of the disease,but did not reach significant level.Conclusion:Flow cytometric measurement of in vivo platelet activation,characterized by its rapidity,reliability,high sensitivity and specificity,is of value in evaluation of in vivo platelet functions in patients with CHD and is able to provide useful data for diagnosis,treatment and prognosis.

关 键 词：血小板活化 流式细胞术 冠心病 

分 类 号：R541.4[医药卫生—心血管疾病] R446.113[医药卫生—内科学]