电针减轻局灶性脑缺血大鼠脑组织病理损伤的作用机理研究  

The Mechanism of Electro-acupuncture in Attenuating Pathological Injury of Brain Tissue in Rats with Focal Ischemia

作　　者：黄浏姣[1] 陈邦国[1] 洪亚群[1] 

机构地区：[1]湖北中医药大学,湖北武汉430061

出　　处：《中医药导报》2013年第1期12-13,16,共3页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：教育部高等学校博士学科点专项科研基金(20094230110002)

摘　　要：目的:探讨电针疗法减轻缺血脑组织病理损伤的可能作用机理。方法:改良线栓法制备局灶性脑缺血再灌注模型,电针"百会"、"水沟"、"足三里",通过逆转录聚合酶链反应(RT-PCR)法半定量测定各组Survivin mRNA的表达。结果:电针组病理改变较相同时相模型组病理改变较轻。相同时相电针组Survivin mRNA的表达比模型组均要高,差异均有统计学意义(P<0.05)。结论:电针疗法可有效减轻缺血脑组织的病理损伤,其作用机理可能与增加缺血脑组织Survivin mRNA的表达有关。Objective： To explore possible mechanism of electro-acupuncture therapy on reducing ischemic brain injury. Methods： Modified Suture-occluded method was applied for forming focal cerebral ischemia reperfusion model; the acupuncture points of Electro-acupuncture were ＂Baihui＂, ＂Shuigou＂ and ＂Zusanli＂; reverse transcriptase polymerase chain reaction （RT- PCR） method was used for semi quantitative determination of serum Survivin mRNA expression. Results： The pathological change in the treatment group was lighter than that model group in the same phase; in the same phase, the Survivin mRNA expression of acupuncture group were higher than that of the model group, with statistically significant differences （P〈0.05）. Conclusion： Electro-acupuncture therapy can provide effect of reducing ischemic brain injury, the mechanism of which may be associated with the increase of Survivin mRNA expression in ischemic brain tissue.

关 键 词：缺血再灌注 电针 SURVIVIN mRNA RT—PCR 

分 类 号：R285.5[医药卫生—中药学]