高胆固醇血症对离体兔主动脉血管内皮依赖性舒张反应的影响  

Hypercholesterolemia Effects on Endothelium Dependent Vasorelaxation

作　　者：王景全[1] 白小涓[1] 张砚[1] 于雅媛[1] Julien I.E.Hoffman 

机构地区：[1]中国医科大学第一医院循环内科,辽宁省沈阳市110001 [2]CardiovascularResearchInstitute,UniversityofCalifornia

出　　处：《中国循环杂志》2000年第3期177-178,共2页Chinese Circulation Journal

摘　　要：目的 :探讨高胆固醇血症对血管内皮依赖性舒张反应的影响。　　方法 :2 0只新西兰雄性兔随机分为两组 :正常对照组 (10只 )及高胆固醇血症组 (10只 )。4周后取出每只兔的降主动脉 ,5 mm宽的动脉环放置于含有 2 5 ml Kreb液的组织—器官水浴系统中。分别测量游离血管对乙酰胆碱 (10 - 1 0 ～ 10 - 5mol/ L )和腺苷 (10 - 1 0 ～ 10 - 4 mol/ L )的舒张反应变化 (% )。　　结果 :两组血胆固醇水平有显著差异 (正常对照组 0 .78± 0 .2 9mmol/ L ,高胆固醇血症组 2 5 .6 7± 2 .86 mmol/ L ,P<0 .0 1) ;高胆固醇血症组血管对乙酰胆碱舒张反应〔最大 (5 8.2± 6 .1) %〕与正常对照组〔最大 (10 3.2± 6 .9) %〕比较明显减弱 (P<0 .0 1) ;高胆固醇血症组血管对腺苷舒张反应〔最大 (4 5 .2± 13.0 ) %〕也较正常对照组〔最大 (10 3.2± 9.9) %〕减弱 (P<0 .0 1)。两组动脉组织学检查无动脉硬化的改变。　　结论 :高胆固醇血症降低血管内皮依赖性舒张反应 ;高胆固醇血症时血管内皮功能改变早于动脉粥样硬化的结构改变。Objective:To determine if hypercholesterolemia effects on endothelial function. Methods:Twenty New Zealand male rabbits were randomly divided into two groups(10 rabbits each):normal diet(NC) and 0 5% cholesterol diet(HC).Four weeks later,descending aorta was excised from each animal,5mm wide aortic rings were placed vertically between hooks in tissue organ bath system containing 25ml Kreb solution.Vasodilator response to acetylcholine (10 -10 -10 -5 mol/L)and adenosine (10 -10 -10 -4 mol/L)was measured in isolated aortic rings.Paired studies were conducted in the hypercholesterolemia and control rabbits. Results:There was a significant difference in total serum cholesterol levels between NC and HC(0 78±0 29 mmol/L and 25 67±2 86 mmol/L, p <0 01);vessels exhibited decreased vosadilator response to acetylcholine〔maximal(58 2±6 1)% in HC and (103 2±6 9)% in NC, p <0 01〕and to adenosine 〔maximal(45 2±13 0)% in HC and (103 2±9 9)% in NC, p <0 01〕,no aortic atherosclerosis was found by morphogical studies. Conclusions:Hypercholesterolemia decreases endothelium dependent vasorelaxation.Vascular endothelial dysfunction develops earlier than atherogenesis in this rabbit model.

关 键 词：高胆固醇血症 内皮细胞功能 舒张反应 

分 类 号：R589.2[医药卫生—内分泌]