哇巴因的升压作用及哇巴因抗体对高血压大鼠血压的影响  

The Pressor Reaction of Ouabain and the Effect of Anti Ouabain Antibody on Blood Pressure of Hypertensive Rats

作　　者：原卫清[1] 王颢[1] 吕卓人[1] 袁育康[2] 任会勋[2] 

机构地区：[1]西安医科大学第一附属医院心内科,陕西省西安市710061 [2]西安医科大学免疫学教研室

出　　处：《中国循环杂志》2000年第3期184-186,共3页Chinese Circulation Journal

基　　金：国家自然科学基金! (No.39670 32 5);国家医药技术创新博士项目! (98- B- 0 7)资助课题

摘　　要：目的 :探讨内源性哇巴因在高血压发病中的作用。　　方法 :分别给予正常 SD大鼠每天腹腔注射外源性哇巴因 (2 5μg/kg,哇巴因组 )及等量生理盐水 (对照组 ) ,观察用药10周血压的动态变化 ;“一肾一夹”高血压大鼠分别给予舌下静脉注射哇巴因抗体 (哇巴因抗体组 )、生理盐水 (生理盐水组 )及正常兔免疫球蛋白 G(正常兔 Ig G组 ) ,颈动脉插管监测血压 ,观察用药后 3小时内血压的动态改变。　　结果 :与对照组比较哇巴因组大鼠注射哇巴因后 3周血压即开始升高 [12 0 .5± 15 .4mm Hg比 10 9.4± 11.8mm Hg(1mm Hg=0 .133 k Pa) ,P<0 .0 5 ],第 10周时血压明显高于对照组 (143.6± 17.3 mm Hg比 12 1.3± 15 .3 mm Hg,P<0 .0 1) ;哇巴因抗体对“一肾一夹”高血压大鼠具有明显的降压作用 ,而正常兔 Ig G组与生理盐水组比较降压作用不明显。　　结论 :体内哇巴因含量升高可能是高血压发病因素之一 ,在血压升高中发挥着较为重要的作用。Objective:To investigate the role of endogenous ouabain (EO) in the pathogenesis of hypertension. Methods:①Sprague dawley rats,which were divided into 2 groups,were injected with ouabain〔25 μg/(kg·day), i.p. 〕or normal saline once a day for 10 weeks,respectively.Systolic blood pressure was recorded weekly.②1klc (one kidney,one clip) hypertensive rats were randomly divided into 3 groups and injected with anti ouabain antibody,normal rabbit immunoglobulin G and normal saline from sublingual vein,respectively.The direct systolic blood pressure of all the rats were recorded from carotid artery before and after injection for 3 hours. Results:①After 3 weeks,blood pressure of rats received ouabain increased significantly and was much higher than those of rats injected with normal saline at the end of week 10.②Anti ouabain antibody could significantly decrease the blood pressure of 1klc hypertensive rats,while the normal rabbit immunoglobulin G or normal saline could not. Conclusions:EO might play an important role in the pathogenesis of hypertension.

关 键 词：哇巴因 高血压 哇巴因抗体 发病因素 

分 类 号：R544.1[医药卫生—心血管疾病]