Selective micellar electrokinetic chromatographic method for simultaneous determination of some pharmaceutical binary mixtures containing non-steroidal anti-inflammatory drugs  

作　　者：Michael E.El-Kommos Niveen A.Mohamed Ahmed F.Abdel Hakiem 

机构地区：[1]Department of Pharmaceutical Analytical Chemistry,Faculty of Pharmacy,Assiut University

出　　处：《Journal of Pharmaceutical Analysis》2013年第1期53-60,共8页药物分析学报（英文版）

摘　　要：A simple and selective micellar electrokinetic chromatographic （MEKC） method has been developed for the analysis of five pharmaceutical binary mixtures containing three non-steroidal antiinflammatory drugs （NSAIDs）. The investigated mixtures were Ibuprofen （IP）-Paracetamol （PC）, Ibuprofen （IP）-Chlorzoxazone （CZ）, Ibuprofen （IP）Methocarbamol （MC）, Ketoprofen （KP） Chlorzoxazone （CZ） and Diclofenac sodium （DS）-Lidocaine hydrochloride （LC）. The separation was run for all mixtures using borate buffer （20 mM, pH 9） containing 15% （v/v） methanol and 100 mM sodium dodecyl sulphate （SDS） at 15 kV and the components were detected at 214 nm. Different factors affecting the electrophoretic mobility of the seven investigated drugs were studied and optimized. The method was validated according to international conference of harmonization （ICH） guidelines and United States pharmacopoeia （USP）. The method was applied to the analysis of five pharmaceutical binary mixtures in their dosage forms. The results were compared with other reported high performance liquid chromatographic methods and no significant differences were observed.A simple and selective micellar electrokinetic chromatographic （MEKC） method has been developed for the analysis of five pharmaceutical binary mixtures containing three non-steroidal antiinflammatory drugs （NSAIDs）. The investigated mixtures were Ibuprofen （IP）-Paracetamol （PC）, Ibuprofen （IP）-Chlorzoxazone （CZ）, Ibuprofen （IP）Methocarbamol （MC）, Ketoprofen （KP） Chlorzoxazone （CZ） and Diclofenac sodium （DS）-Lidocaine hydrochloride （LC）. The separation was run for all mixtures using borate buffer （20 mM, pH 9） containing 15% （v/v） methanol and 100 mM sodium dodecyl sulphate （SDS） at 15 kV and the components were detected at 214 nm. Different factors affecting the electrophoretic mobility of the seven investigated drugs were studied and optimized. The method was validated according to international conference of harmonization （ICH） guidelines and United States pharmacopoeia （USP）. The method was applied to the analysis of five pharmaceutical binary mixtures in their dosage forms. The results were compared with other reported high performance liquid chromatographic methods and no significant differences were observed.

关 键 词：CAPILLARYELECTROPHORESIS Micellar electrokineticchromatographicmethod Non-steroidalanti-inflammatorydrugs Pharmaceutical binarymixtures Pharmaceutical analysis 

分 类 号：R927.2[医药卫生—药学]