脑缺血再灌注后大鼠海马Wnt7b的表达  

Expression and location of Wnt7b in rat subgranular zone of hippocampus dentate gyrus after cerebral ischemia-reperfusion

作　　者：李慧[1] 黄景阳[1] 陈海丽[1] 刘宝义[2] 袁中瑞[1] 

机构地区：[1]山东大学医学院病理学与病理生理学教研室,济南250012 [2]山东大学齐鲁医院呼吸内科,济南250012

出　　处：《山东大学学报（医学版）》2013年第2期7-11,共5页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81171106);山东省自然科学基金(2009ZRB01125);中国博士后科学基金(20110491578);山东省博士后创新基金(201102016)

摘　　要：目的研究成年大鼠脑缺血再灌注后海马齿状回颗粒细胞下层(SGZ)Wnt7b的表达。方法将44只雄性Wistar大鼠随机分为正常对照组和脑缺血再灌注组,线栓法制备大鼠脑缺血再灌注损伤模型,缺血90 min,术后检测神经功能缺失和脑梗死,在脑缺血再灌注后1、3、7、14 d取大鼠海马组织。RT-PCR技术检测Wnt7b mRNA表达水平;免疫荧光技术检测海马Wnt7b蛋白及β-catenin蛋白表达水平。结果脑缺血后,大鼠出现严重的脑梗死和神经功能障碍,随再灌注时间延长,大鼠神经功能得到修复。再灌注7d后,健侧脑组织海马Wnt7b mRNA表达上调(P<0.05),缺血侧脑组织海马Wnt7b mRNA的表达明显上调(P<0.01)。Wnt7b主要分布于SGZ附近,阳性细胞增多(P<0.01),且该脑区的细胞内β-catenin阳性细胞数目增多(P<0.01)。结论缺血性脑损伤可刺激Wnt7b在成年海马SGZ的表达上调,Wnt7b可能通过经典Wnt通路参与调节脑缺血后成年海马SGZ的神经发生。Objective To investigate the involvement of Wnt in ischemia-induced adult nerve regeneration and explore the mechanism of the two members. Methods 44 normal male Wistar rats were randomly divided into 2 groups： the normal control group and the cerebral ischemia group. Cerebral ischemia-reperfusion rat models were established by inserting a proper thread in the right middle cerebral artery and removing the thread after 90 min. Infarction and neurological deficit scores were evaluated after the operation. RT-PCR was used to investigate the expression of Wnt7b mRNA in hippocampus. Immunohistochemistry was used to investigate expressions of Wnt7b and 13-catenin proteins in the dentate gyrus of hippocampus. Results Severe infarction appeared 1 day after ischemia-reperfusion, with obvious neurological deficit. Neural function＇s were improved as time progressed. Expressions of Wnt7b mRNA in health side and ischemic side were significantly upregulated, and it was more obvious in ischemic side （ P 〈 0.01 ） than in contralateral side（ P 〈 0.05 ）. Consistent with the level of Wnt7b mRNA, the amount of Wnt7b positive cells dramatically increased in close proximity to the subgranular zone （SGZ） of the dentate gyrus in hippocampus（P 〈 0.01 ）, and the immunostaining of β-catenin, a primary element of classical Wnt signalling pathway, also significantly increased （P 〈 0.01 ）. Conclusion Ischemia injury stimulated the expressions of Wnt7b and β-catenin in SGZ of hippocampus in adult rats. Wnt7b might be involved in the neurogenesis in SGZ of adult hippocampus after cerebral ischemia by the classical Wnt signalling pathway.

关 键 词：脑缺血 基因 Wnt7b Β-CATENIN蛋白 海马 神经发生 大鼠 Wistar 

分 类 号：R614.2[医药卫生—麻醉学] R332[医药卫生—外科学]