绵羊发情周期不同组织Cry1 mRNA转录水平相对定量研究  

Relative quantification of mRNA transcription of Cry1 in different tissues of sheep in oestrous cycle by real-time quantitative PCR

作　　者：高磊[1,2] 甘尚权[1,2] 杨井泉[1,2] 杨剑波[1,2] 梁耀伟[1,2] 阿不都艾尼.努拉洪 沈敏[1,2] 

机构地区：[1]新疆农垦科学院畜牧兽医研究所,石河子832000 [2]新疆兵团绵羊繁育生物技术重点实验室,石河子832000 [3]新疆兵团农三师四十四团兽医站,图木舒克市843900

出　　处：《遗传》2013年第1期85-92,共8页Hereditas（Beijing)

基　　金：973计划前期研究专项(编号:2009CB125907);国家自然科学基金项目(编号:30960249);兵团种质资源创新与功能基因发掘及利用专项(编号:2012BB044)资助

摘　　要：研究表明,时钟基因Cry1在哺乳动物季节性繁殖内分泌过程中可能发挥了重要作用。文章以多浪羊(非季节性繁殖)和中国美利奴羊(季节性繁殖)为研究对象,采用实时荧光定量PCR技术对下丘脑-垂体-性腺轴主要组织Cry1在发情周期不同阶段的表达变化情况进行了跟踪检测。结果表明:绵羊Cry1在所检测组织中均有表达,其中松果体和甲状腺Cry1的表达水平较高;不同绵羊品种Cry1在发情周期不同阶段的组织表达谱基本一致,除下丘脑外,卵巢、子宫、松果体、垂体和甲状腺中Cry1的表达水平均是在发情前期达到峰值;卵巢、子宫、垂体和松果体Cry1在发情前期和发情期的表达变化幅度存在显著的品种间差异。研究结果表明:Cry1可能具有启动发情和季节性繁殖的重要作用。Studies have shown that clock gene Cry1 may have important roles in the endocrine process of seasonal reproduction in mammals. In this study, Duolang sheep （non-seasonal reproduction sheep breed） and Chinese Merino （seasonal reproduction sheep breed） were used to determine the expression change of Cry1 in hypothalamus-pituitary-ovary axis in different stage of estrous cycle by quantitative real-time PCR. The results showed that the Cry1 mRNA was expressed in all tested tissues, in which the expression levels in pineal gland and thyroid gland were higher than in other tissues. As far as different sheep breeds were concerned, the tissue expression profiles of Cry1 at different stage of estrous cycle were broadly similar. Besides hypothalamus, the expression levels of Cry1 in ovary, uterus, pineal gland, pituitary gland, and thyroid gland were all reached to peak in proestrus. The differences of expression change extent for Cry1 in vary, uterus, pineal gland, and pituitary gland in proestrus and oestrus were significant between different sheep breeds. The results suggested that Cry1 may play roles in switching on the estrus and seasonal reproduction.

关 键 词：Cry1基因 发情周期 下丘脑-垂体-性腺轴 绵羊 

分 类 号：S823[农业科学—畜牧学]