基于线粒体12S基因对青藏高原细粒棘球蚴种群遗传结构的研究  

Population genetic structure of Echinococcus granulosus in Qinghai-Tibetan Plateau based on mtDNA 12S gene

作　　者：延宁[1] 聂华明[1] 蒋忠荣[2] 邓世金 余华[3] 严玉宝[3] 达瓦次仁 孔维淑 谢跃[1] 付彦[1] 杨德英[1] 古小彬[1] 杨光友[1] 

机构地区：[1]四川农业大学动物医学院,雅安625014 [2]四川省甘孜州畜牧科学研究所,康定626000 [3]四川出入境检验检疫局,成都610041 [4]西藏自治区动物疫病预防控制中心,拉萨850000 [5]西藏那曲地区职业技术学校,那曲852000

出　　处：《中国人兽共患病学报》2013年第1期10-16,共7页Chinese Journal of Zoonoses

基　　金：国家科技支持计划项目(2006BAI06B09);国家质检总局科技计划项目(2009IK019)~~

摘　　要：目的分析青藏高原细粒棘球蚴的种群遗传结构,为细粒棘球蚴病的防控提供基础资料。方法对采自青藏高原来自不同地区(青海、西藏、四川)不同宿主(人、绵羊、牛)的58株细粒棘球蚴进行了线粒体12S基因的全序列测序,结合已报道的青海地区42个细粒棘球蚴绵羊株的12S全序列进行种群遗传结构分析。结果本次研究56个分离株鉴定为Echino-coccus.granulosus sensu stricto(基因型G1–G3);分析98个青藏高原地区分离株12S序列,包含15个核苷酸变异位点,共检测出13个单倍型(ES1～ES13),单倍型多样性(Hd)和核苷酸多样性(Pi)分别为0.381、0.00092。3个地域种群中,四川种群的核苷酸多样性最高(0.001353),种群内遗传距离最大(0.00135)。分子变异分析(AMOVA)显示,种群内的遗传变异大于种群间的遗传变异。构建单倍型NJ树和贝叶斯树显示,ES10和ES11形成一个有别于其他单倍型的单倍型类群。单倍型网络显示,单倍型ES1是优势单倍型,其它单倍型围绕它成辐射状,未发现有地理聚类。基因流(Nm值)和遗传分化指数(FST值)显示各地域种群间未形成显著的遗传分化。结论青藏高原细粒棘球绦虫的基因型为G1-G3型;青藏高原细粒棘球绦虫种群内及种群间都存遗传多样性,其中四川种群的遗传变异性最大;青藏高原细粒棘球绦虫种群内变异是种群变异的主要来源;种群未形成地理聚类,遗传分化不明显。The aim of the current study is to investigate the population genetic structure of Echinococcus granulosus （E. granulosus） obtained from Qinghai-Tibetan Plateau, and provide the foundational data for prevention and control of echi- nococcal disease. The complete sequences of mitochondrial DNA 12S genes of 98 isolates from different areas （including Qing- hal, Tibet and Sichuan populations） and different hosts （such as human being, sheep, or cattle） were sequenced and combined with forty-two 12S sequences of Qinghai sheep strains which had reportedly analyzed. The result of sequences analysis was thatall 56 isolates were identified to be E. granulosus sensu stricto （genotype G1-G3）. Of 98 strains were classified into 13 hap- lotypes （ES1-ES13）, while 15 nucleotide variation sites were detected. Haplotypes diversity and nucleotide diversity were 0. 381 and 0. 00092, respectively. The nucleotide diversity （0. 001353） and genetic distance （0. 00135） of Sichuan popu lation were the highest one in the three populations. It was showed by AMOVA that 97.36% of the total genetic variabil-ity was found among haplotype groups. NJ tree and Mrbayes tree showed that ES10 and ES11 formed a haplotype group which was different from other haplotypes. Network showed that ES1 was the main haplotype, and others centered to it, without any geographic cluster finding. The points of Nm and Fsr showed that there was not significant genetic differentiation among popu- lations. The result showed that E. granulosus of genotype G1-G3 were found in Qinghai-Tibetan Plateau. Genetic diversity ex- isted in and among the populations of Qinghai-Tibetan Plateau, and the genetic diversity of Sichuan population was higher than that of other populations. The variation which happened in the population leads to the intraspecific variation. Geography cluster has not been found, and the genetic differentiation is not significant.

关 键 词：细粒棘球蚴 12S基因 种群遗传机构 青藏高原 

分 类 号：S855.9[农业科学—临床兽医学]