福建省MSM人群中HIV-1感染者原发性耐药基因突变研究  

Primary drug-resistant gene mutation to HIV-1 infected MSM in Fujian Province

作　　者：谢关榕[1] 严延生[1,2] 颜苹苹[1,2] 吴守丽[1,2] 陈舸[1,2] 张春阳[1] 

机构地区：[1]福建省疾病预防控制中心,福建省人兽共患病研究重点实验室,福州350001 [2]福建医科大学公共卫生学院,福州350001

出　　处：《中国人兽共患病学报》2013年第1期27-29,42,共4页Chinese Journal of Zoonoses

基　　金：福建省自然科学基金(2009J01104)~~

摘　　要：目的研究我省男男性行为者(MSM)中未经抗病毒治疗的人类免疫缺陷病毒(HIV)感染者的原发性基因耐药性突变发生情况,为该人群的抗病毒治疗药物选择提供依据。方法采集福建省尚未接受抗病毒治疗的MSM HIV-1感染者的外周静脉抗凝全血,分离血浆,提取血浆中HIV RNA,用RT-PCR方法扩增HIV pol区蛋白酶基因全序列与部分逆转录酶序列。所得扩增片段进行序列测定后,数据运用Contig Express拼接编辑校正后上传至斯坦福大学HIV耐药数据库进行比对,同时运用Bio-Edit和Mega等软件进行系统发生分析。结果获得50份HIV-1分离株完整的PR和RT基因序列,其中60%(30/50)为01_AE重组亚型,18%(9/50)为B亚型,20%(10/50)为07_BC重组亚型,1例08_BC重组亚型。耐药分析显示,1例发生PI(蛋白酶抑制剂)药物的主要耐药突变M46I,造成对NFV中度耐药。10例样本出现NNRTIs和NRTIS的耐药相关突变,仅1例解释为对NNRTIs的潜在低水平耐药,由V179E引起。结论福建省MSM HIV-1感染者的原发性耐药突变发生率低,有利于今后该人群抗病毒治疗工作的开展,但需加强监控该人群耐药毒株的传播。The purpose of the present study is to investigate the HIV-1 drug-resistant gene mutation rate on protease and reverse transcriptase in HIV infected men who have sex with men （MSM） without antiretroviral therapy （ART） in Fujian Province in order to provide evidences for selection of appropriate ART drugs. HIV-1 viral RNA were extracted from plasma samples and then the whole pol gene and partial reverse transcriptase gene were amplified by reverse transcription polymerase chain reaction （RT-PCR）. The sequences of those amplified fragments were edited using the software Contig Express and sub- mitted to the website http：//hivdb, stanford, edu/ for analysis of their drug resistance status. Meanwhile, their phylogenesis was analyzed by Bio-Edit, Clustal, and Mega. It was found that 60% （30/50） of that strains were classified as CRF01_AE, 10 were CRF 07_BC, 9 were B and one was CRF 08_BC. According to the interpretation of Stanford University drug resistance database, 5 of 50 sequences occurred mutations for PIs, only one with M46I mutation was interpreted as intermediate resistant to NFV. Of 10 sequences occurred mutations for NNRTIs （non-nucleoside reverse transcriptase inhibitors） and NRTIs （nucle- oside reverse transcriptase inhibitors）, and the one carried V179E was interpreted as potential low-level resistance to NNRTIs. These results reveal that although the low mutation rate was showed in MSM HIV-1 infected patients in Fujian Province, the surveillance for the transmission of drug-resistant HIV-1 in the target population is necessary.

关 键 词：人类免疫缺陷病毒 男男性行为者 耐药 基因突变 

分 类 号：R373.9[医药卫生—病原生物学]