维生素E对约氏疟原虫感染BALB/c小鼠早期免疫应答的抑制效应  

Vitamin E inhibits immune responses during the early stage of P.y 17XL infection in BALB/c mice

作　　者：延娟[1] 刘军[1] 李莹[1] 潘艳艳[1] 郑丽[1] 曹雅明[1] 

机构地区：[1]中国医科大学基础医学院免疫学教研室,沈阳110001

出　　处：《中国免疫学杂志》2012年第12期1125-1128,共4页Chinese Journal of Immunology

摘　　要：目的:探讨维生素E(Vitamin E,VE)对致死型约氏疟原虫(Plasmodium yoelii 17XL,P.y17XL)感染BALB/c小鼠的免疫调节效应。方法:6～8周、雌性BALB/c小鼠,随机分为实验组和对照组,经腹腔接种1×106P.y17XL寄生的红细胞,实验组于感染前10天口服给予VE预处理。动态观察两组小鼠的原虫血症水平;流式细胞术检测感染后第0、3和5天脾树突状细胞(Dendritic cells,DCs)亚群和Toll样受体9(Toll like receptor,TLR9)表达水平、以及巨噬细胞和活化T细胞的表达数量。结果:(1)与正常感染组相比较,补充VE加重感染小鼠的原虫血症水平,死亡率于感染后第6天达到70%,而正常感染组在感染后第6天死亡率只有30%;(2)与正常感染组相比,在每一检测点,VE处理组小鼠脾脏DCs亚群表达水平显著降低;同时,F4/80+巨噬细胞数量明显低于对照组,在感染后第5天,VE处理组小鼠脾脏T细胞活化水平显著降低。结论:在P.y17XL感染早期,VE预处理可通过抑制DCs的成熟和分化,从而加剧P.y17XL感染BALB/c小鼠的感染进程。Objective：To investigate the immunoregulatory effect of VitaminE（VE） on BALB/c mice infected with Plasmodium yoelii 17XL（P.y17XL）.Methods：Female 6–8-week-old BALB/c mice were randomly divided into two groups.Mice were orally administered VE 10 days before P.y17XL infection,once a day.Parasitemia was monitored by light microscope examination of Giemsa stained thin（tail） blood smears.We tested the subsets of splenic dendritic cells（DCs）：CD11c＋CD11b＋ myeloid DCs（mDCs） and CD11c＋CD45R＋/B220＋ plasmacytoid DCs（pDCs）,the expressions of Toll-like receptor 9（TLR9） in CD11c＋ DCs,the percentage of macrophages and CD4＋CD69＋ T cells on day 3,5 post infection（p.i.） by flow cytometry.Results：（1） Pretreatment with VE increased the parasitemia and the mortality rate was 70% on day 6（p.i）,but the control group was only 30%.（2） the subsets of splenic DCs and the number of F4/80＋ macrophages was significantly reduced in VE group;meanwhile,the activation of CD4＋ T cells was declined on day 5（p.i）.Conclusion：VE pretreatment aggravate the progress of the malaria infection by inhibiting the mature and differentiation of DCs during the early stages of P.y17XL infection.

关 键 词：VE 致死型约氏疟原虫 固有免疫 免疫抑制 

分 类 号：R328.3[医药卫生—人体解剖和组织胚胎学]