青藏高原冻土链霉菌分离菌株多样性及其生理活性的研究  

Diversity and Physiological Aactivity of Streptomyces spp.Isolated from Permafrost of the Qinghai-Tibetan Plateau

作　　者：马爱爱[1] 张新芳[1,2] 赵林[2] 徐世健[1,2] 敏玉霞[1] 王鹏[1] 程国栋[2] 

机构地区：[1]兰州大学生命科学学院,甘肃兰州730000 [2]中国科学院寒区旱区环境与工程研究所,甘肃兰州730000

出　　处：《冰川冻土》2012年第6期1508-1516,共9页Journal of Glaciology and Geocryology

基　　金：国家自然科学基金项目(40871041;40671042);甘肃省科技支撑计划项目(1011FKCA142);科技部基础性工作专项“青藏高原多年冻土本底调查”(2008FY110200);中央高校基本科研业务费专项(lzujbky-2011-118)资助

摘　　要：以青藏高原腹地北部地区5个钻孔中采集的冻土样品为材料,采用高氏1号琼脂和甘油精氨酸琼脂两种培养基,分离得到54株可培养放线菌.经形态学初步鉴定并结合16SrDNA序列分析,将分离菌株归入链霉菌属.抑菌实验和生理生化特性研究发现,24%的链霉菌对供试病原菌有抑菌活性,H2S、有机酸、H2O2酶、脂酶Ⅰ(Tween-20)、脂酶Ⅱ(Tween-40)、脂酶Ⅲ(Tween-80)、淀粉酶、蛋白酶、脲酶产生菌分别占分离菌株的56%、28%、93%、22%、67%、41%、43%、61%、57%.结果显示,青藏高原冻土蕴藏有丰富的放线菌资源,其次级代谢产物的丰富性和多样性,对开发研究放线菌资源具有重要价值.Ten soil samples were collected from five boreholes in the northern part of Qinghai-Tibetan Plateau. Actinomycetes isolated using two mediums were identified as Streptomyces spp. according to their morphological characteristics in combination with full 16S rDNA sequencing. It is found from antimicrobial and physiological studies that 24 % of the total isolates have antimicrobial activity to the target pathogens. H2S, organic acid, catalase, esterase I （Tween-20）, esterase Ⅱ （Tween 40）, esteraseⅢ （Tween-80）, amylase, proteinase, urease producing strains account for 56%, 28%, 93%, 22%0, 67% , 41%, 43%, 61% and 57% of the total isolates, respectively. The abundant physiological diversity and secondary metabolites of streptomycetes isolated from permafrost of the Tibetan Plateau may have some potential perspecrive of biotechnology biological products.

关 键 词：青藏高原 冻土 放线菌 多样性 生理活性 

分 类 号：Q938[生物学—微生物学]