猪去细胞主动脉瓣组织学及生物力学特征  

Histology and physicochemical properties of porcine valve tissue with cells extracted

作　　者：金振晓[1] 刘维永[1] 

机构地区：[1]第四军医大学西京医院心血管外科中心,陕西西安710033

出　　处：《第四军医大学学报》2000年第5期592-594,共3页Journal of the Fourth Military Medical University

摘　　要：目的　观察猪主动脉瓣叶去细胞后的组织学和理化性能变化 ,探讨其作为未来“杂种”瓣膜支架的可能性 .方法　新鲜采集的猪主动脉瓣叶经表面活性剂、核酸酶、渗透压变化作用 ,去除瓣叶组织的细胞成分 ,在光镜、电镜下观察组织学变化 ,测定瓣叶组织的含水量、可溶性蛋白含量、热皱缩温度、瓣叶厚度、描计了应力应变曲线 .结果　成功地去除了猪主动脉瓣组织中的细胞成分 ;瓣叶去细胞后组织含水量明显增高 [φ( 94.6± 0 .3) % vs ( 91.2± 2 .0 ) % ,P<0 .0 1],可溶性蛋白含量明显降低 [ω( 0 .0 41± 0 .0 0 8) % vs( 0 .0 5 6±0 .0 0 6 ) % ,P<0 .0 5 ];热皱缩温度 [( 72 .0± 0 .7)℃ vs( 71.2±0 .8)℃ ,P>0 .0 5 ],瓣叶厚度 [( 0 .8± 0 .3) m m vs ( 0 .7± 0 .3)mm,P>0 .0 5 ],断裂强度 [( 6 46± 133) g.mm- 2 vs( 6 5 0±10 5 ) g.mm- 2 ,P>0 .0 5 ]和断裂伸长率 [( 5 7± 14) % vs( 6 5±18) % ,P>0 .0 5 ]均无明显差别 .结论　采用表面活性剂、核酸酶结合渗透压变化的方法 ,能够成功地去除猪主动脉瓣组织的细胞成分 ,且不影响组织的理化性能 ,有可能作为纤维支架 ,用于构建“杂种”AIM To observe the heart valve tissue with cells extracted and to explore its possibility to serve as a scaffold in constructing a 'hybrid' heart valve prosthesis. METHODS The fresh porcine aortic heart valves were treated with triton X100, RNAase and DNAase solution combined withalterations of osmosis to remove all the cellular components. Light and electron microscopy was used to investigate the histological changes. The soluble protein content, water content, shrinkage temperature, tension and strain at fracture of fresh and acellular valve tissue were evaluated. RESULTS Light and electron microscopy confirmed that all the cellular constituents were removed without ultrastructural damage to fibrous components. Compared with the fresh sample, the water content of acellular tissue increased significantly [φ(94.6±0.3)% vs (91.2±2.0)%, P<0.01]; the soluble protein content decreased markedly [ω(0.041±0.008)% vs (0.056±0.006)%, P<0.05]. As to other indexes evaluated, no significant difference was found between acellular and fresh valve tissues: Shrinkage temperature [(72.0±0.7)℃ vs ( 71.2 ±0.8)℃, P>0.05]; thickness [(0.8±0.3) mm vs (0.7±0.3) mm, P>0.05]; tension at fracture [(646±133) g/mm2 vs (650±105) g/mm2, P>0.05]; strain at fracture [(57±14)% vs (65±18)%, P>0.05]. CONCLUSION The cellular component of fresh porcine aortic heart valves can completely be removed without impairing the mechanical property of the tissue. This kind of acellular tissue, therefore, could possibly be used to develop tissue engineering for heart valve substitute.

关 键 词：生物瓣 组织学 异种生物瓣 生物力学 

分 类 号：R654.2[医药卫生—外科学] R318.11[医药卫生—临床医学]