肢体负压对周围动脉闭塞犬皮肤CGRP免疫反应性神经的影响  

Influence of limb negative pressure on cutaneous CGRP immunoreactive nerve fibers in dogs of peripheral arterial occlusive disease

作　　者：易军[1] 宁莫凡[1] 邱建勇[2] 

机构地区：[1]第四军医大学西京医院血管外科,陕西西安710033 [2]第四军医大学,陕西西安710033

出　　处：《第四军医大学学报》2000年第5期639-641,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目!(39700122)

摘　　要：观察肢体负压对周围动脉闭塞性病变犬皮肤中 CGRP免疫反应阳性神经纤维的影响 .方法　犬 17只 ,随机分治疗组 9只、非治疗组 5只和正常对照组 3只 ,治疗组和非治疗组均将动物制作左后肢缺血模型 ,治疗组在模型制作后14d,开始行患肢负压治疗 10 d ( 15 m in/次 ) ;非治疗组不做负压治疗 .3组均行左后肢趾皮肤免疫组化染色 ,检测 CGRP免疫反应阳性纤维 .结果　非治疗组皮肤中 CGRP免疫反应阳性神经纤维较正常对照组明显增多 ( P<0 .0 1) ,治疗组较非治疗组减少 ( P<0 .0 1) ,但仍较正常对照组增多 .结论　提示肢体负压疗法可促进皮肤感觉神经纤维中AIM To observe the influence of limb negative pressure (LNP) on cutaneous CGRP immunoreactive nerve fibers in dogs with peripheral arterial occlusive disease. METHODS Seventeen dogs were divided into treatment (n=9), nontreatment (n=5) and normal control (n=3) groups randomly. The left hindlimb ischemia model was made in nontreatment and treatment groups. The treatment group was treated by LNP for ten days (15 min/day) from the 14th day after the models were made. However, the nontreatment group was not treated. All the animals were detected for the change of CGRP immunoreactive nerve fibers in the skin of ischemia toes. RESULTS The CGRP immunoreactive nerve fibers in the skin of nontreatment group were increased more than those in the normal control group (P< 0.01 ). The CGRP immunoreactive nerve fibers of the treatment group were decreased than those in the nontreatment group (P<0.01) but more than those in normal control group. CONCLUSION The LNP might promote the releasing of CGRP in the cutaneous sensory nerve fiber endings.

关 键 词：CGRP 免疫组织化学 周围动脉闭塞性 肢体负压 

分 类 号：R654.3[医药卫生—外科学] R658[医药卫生—临床医学]