太白楤木薄层色谱鉴别方法研究  

Studies on the Methods to Identify of Aralia taibaiensis by TLC

作　　者：师延琼[1] 王幸[1] 覃鸿恩[1] 崔春利[1] 郭东艳[1] 

机构地区：[1]陕西中医学院药学院,陕西咸阳712046

出　　处：《现代中药研究与实践》2012年第6期16-18,共3页Research and Practice on Chinese Medicines

基　　金：陕西省教育厅资助项目(09JS015;2010JK506);陕西省中管局资助项目(ZY30;ZY31)

摘　　要：目的建立太白楤木根皮的薄层色谱鉴别方法。方法通过对不同制样方法、点样量、展开系统、展距的比较,优化太白楤木根皮的薄层色谱条件。结果最佳色谱条件为:展开剂:氯仿:乙酸乙酯:甲醇:水=15:40:22:10;展距:15cm;温度:15℃左右;湿度:30%～50%;显色方法 :10%硫酸乙醇溶液,105℃加热4 min,置紫外光灯(365 nm)下检视。结论在上述色谱条件下,太白楤木根皮的薄层色谱斑点清晰,信息量较大,且专属性强、重现性好,能有效鉴别太白楤木。Objective To establish the identification method ofAralia taibaiensis by TLC. Methods Aralia taibaiensis TLC conditions were optimized by different sample preparation methods, point-like volume, expand the system. ResμLt The optimum chromatographic conditions were as follows： developing solvent （chloroform： ethyl acetate： methanol： water = 15：40：22： 10）, developing distance 15 cm, temperature 15 ℃, humidity from 30% to 50%, colour-display methods （10% sμLfuric acid in ethanol, 105℃ for 4 minutes, under 365nm UV light）. Conclusion Aralia taibaiensis TLC spots were clear under the above chromatographic conditions which eoμLd identify Aralia taibaiensis effectively.

关 键 词：太白楤木 薄层色谱 鉴别 

分 类 号：R324[医药卫生—人体解剖和组织胚胎学]