维药骆驼刺质量标准研究  

Research on Quality Standard of Ethnic Drug Alhagi Sparsifolia Shap

作　　者：徐晓琴 魏鸿雁 石磊岭 夏提古丽.阿不力孜 贾晓光 

机构地区：[1]新疆维吾尔自治区中药民族药研究所,新疆乌鲁木齐830002

出　　处：《现代中药研究与实践》2012年第6期68-71,共4页Research and Practice on Chinese Medicines

基　　金：新疆乌鲁木齐市科技创新种子资金项目(K111410001);新疆维吾尔自治区科技成果转化专项资金项目(201154129)

摘　　要：目的建立维药骆驼刺的质量标准。方法采用《中华人民共和国药典》2010年版（一部）附录ⅨA杂质检查法、附录ⅨH烘干法和附录ⅨK总灰分测定方法检查药材的杂质、水分和总灰分，采用附录vIB薄层色谱法建立定性鉴别骆驼刺的方法；采用附录vID高效液相色谱法测定药材中芦丁的含量。结果骆驼剃含杂质应不得超过0．5％，水分应不得超过10％，总灰分应不得超过12％；薄层色谱斑点清晰，专属性强；芦丁在5．05—101μg范围内线性关系良好，回归方程：Y=3．26459×10^-8- 1．537145×10^-4，r=0．9999，加样回收率为97．8％，RSD=2．33％（n=6）。结论该方法简便、准确、重复性好，有助于维药骆驼刺的质量控制。Objective To study the quality standards ofAlhagi sparsifolia shap. Methods The impurity limit, moisture content, total ash were determined based on the procedures records in the Appendix IX A, IX H and IX K of pharmacopoeia （published in 2010）. The thin layer chromatography （TLC） method was established. The rutin was determined by HPLC （the Appendix VI D）. ResμLt Impurities were nO more than 0.5%, the moisture content was no more than 10% and the total ash was no more than 12%. The TLC spots were clear and specific, and the rutin showed a good linear relationship at a range of 5.05-101 μg with the regression equation as Y=3.264 59× 10^8-X-1.537 145×10-4, r=0.999 9. RSD=2.33% （n=6）. And the average recovery was 97.8%. Conclusion The method was easy, accurate, quick and reproducible which was benefit for the quality control of Alhagisparsifolia shap.

关 键 词：骆驼刺 质量控制 RP—HPLC色谱法 芦丁 

分 类 号：R917[医药卫生—药物分析学]