HP-PRRS纯化灭活疫苗免疫效力研究  

Study on Immune Effect of HP-PRRS Inactivated Vaccine

作　　者：唐艳林[1] 宋桂才 李国江[1] 何玉友[3] 刘军[3] 王复蒙 

机构地区：[1]吉林农业科技学院动物医学学院,吉林132101 [2]保定瑞普生物药业有限公司,保定071000 [3]吉林正业生物制品有限公司,吉林132101

出　　处：《吉林农业科技学院学报》2012年第4期4-7,共4页Journal of Jilin Agricultural Science and Technology University

基　　金：吉林市重点科技计划项目(项目编号:201022110)

摘　　要：为提高目前高致病性蓝耳病(HR-PRRS)灭活疫苗的免疫效果,将经Marc-145细胞培养收获的HP-PRRS病毒原液超滤浓缩50倍,再经灭活后用Sepharose 4 Fast Flow分子筛柱层析,收集完整的病毒粒子,加油佐剂制成纯化HP-PRRS灭活疫苗。纯化后疫苗的攻毒保护率达到4/5以上,血清抗体检测阳转率达86%以上,均高于同批病毒液制成的未纯化灭活疫苗,且差异显著。试验证明,HR-PRRS病毒液经浓缩纯化制成疫苗后,增强了免疫效果。To improve purification and protective potency of HP- PRRS inactivated vaccine, HP- PRRS vires multiplying and collected from Marc - 145 cells was concentrated 50 times and then inactivated. Complete virion is separated and collected by chromatography with Sepharose 4 Fast Flow. Oil adjuvant was added to prepare purified in- activated vaccine. Protective potency of purified vaccine is above 4/5 and positive conversion rate of antibody is over 86 % that all higher than that of unpurified vaccine. The differences are significant. The experiment illustrated that vaccines through concentrating and purifying can enhanced immune efficacy and non- viral protein can be removed.

关 键 词：HR—PRRS灭活疫苗 浓缩纯化 攻毒保护率 抗体阳转率 

分 类 号：S858.28[农业科学—临床兽医学]