严重颅脑损伤脑组织匀浆诱导人脐血MSCs成神经分化的实验研究  

Study on severe brain injury induced by brain homogenates human umbilical cord blood MSCs into neuronal differentiation

作　　者：吕品[1] 单桂秋[1] 张雅妮[1] 邓均[2] 郑峻松[2] 

机构地区：[1]广州军区广州总医院输血科,广东广州510010 [2]第三军医大学医学检验系临床检验学教研室,重庆400038

出　　处：《国际检验医学杂志》2012年第18期2183-2184,共2页International Journal of Laboratory Medicine

摘　　要：目的探讨严重颅脑损伤时的脑组织匀浆对人脐血间充质干细胞(MSCs)成神经分化的诱导能力。方法制作小鼠严重颅脑损伤模型,取伤后24h和正常小鼠脑组织匀浆,对体外培养的第2代MSCs进行诱导。诱导3d后以荧光免疫细胞组化技术检测细胞内NF和NeuN的表达。结果成功培养出人脐血MSCs,成神经诱导后,严重损伤性脑组织匀浆诱导组NF和Ne-uN阳性率分别为(47.31±8.14)%和(58.91±8.12)%,高于正常脑组织匀浆诱导组阳性率(P<0.05)。结论脑组织匀浆可诱导人脐血MSCs向神经元样细胞分化,表达神经细胞特异标志分子NF和NeuN,严重损伤性脑组织匀浆的诱导效果高于正常脑组织匀浆。Objective To explore serious head injury when the brain homogenates of human umbilical cord blood mesenchymal stem cells（MSCs） into the induction of neuronal differentiation. Methods Production in mice of severe traumatic brain injury mod- el, take the injury after 24 h and normal mice brain tissue homogenates, induced by the 2nd generation of MSCs cultured in vitro. After induction of 3 days,immune cell staining was performed to detect intraeellular expression of NF and NES. Results Human um- bilical cord blood MSCs were successfully cultured. After neural induction,immunocytochemieal analysis showed that positive rates of NF and NeuN of severe traumatic brain homogenates induced group were（47.31 ± 8.14）% and （58.91±8.12）%, significantly higher than those of normal brain tissue homogenate induced group（P〈0.05）. Conclusion Brain homogenates could induce differ- entiation of human umbilical cord blood MSCs to neuron like cells, with expression of NF and NeuN. Induction effects of severe traumatic brain homogenates might be significantly higher than normal brain tissue homogenates.

关 键 词：间质干细胞 胎血 颅脑损伤 神经分化 

分 类 号：R651.1[医药卫生—外科学]