流行性出血热肾组织中增殖细胞核抗原、波形丝蛋白的表达及其意义  

Expression and significance of proliferating cell nuclear antigen and vimentin in renal tissue from the patients with epidemic hemorrhagic fever

作　　者：陈威[1] 杨守京[2] 刘彦仿[2] 徐月清[1] 李元勋[1] 

机构地区：[1]第四军医大学西京医院肾内科,陕西西安710032 [2]第四军医大学病理学教研室,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2000年第3期228-229,231,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助!No.39270290

摘　　要：目的　研究流行性出血热 (EHF)肾组织中增殖细胞核抗原 (PCNA)和波形丝蛋白 (vimentin)的表达及意义。方法　应用多重PAP免疫组化方法 ,对 17例EHF尸检肾组织中PCNA和vimentin的表达进行观察。结果　EHF尸检肾组织PCNA的阳性率为 6 4 71% ,集合管的增殖指数显著大于远曲小管 (P <0 0 1) ,低血压休克期患者远曲小管和集合管上皮细胞vimentin呈强阳性表达 ,相同部位PCNA也呈阳性 ,PCNA的阳性强度与局部病变的程度相一致。结论EHF患者急性肾功能衰竭的早期就存在损伤肾小管上皮细胞的再生、增殖和分化 。Aim To study expression and significance of proliferating cell nuclear antigen(PCNA) and vimentin in renal tissues of the patients with epidemic hemorrhagic fever(EHF). Methods 17 autopsy renal tissues from the patients died of EHF were examined by immunohistochemical staining(PAP). Results The results showed that positive rate of PCNA expression in 17 autopsy renal tissues was 6471% ; proliferative index of collecting tubes was significantly higher than that of distal convoluted tubules(P< 0001); Vimentin expression of epithelial cells in distal convoluted tubules and collecting tubules from the EHF patients at hypotensive shock stage were strongly positive,PCNA expression at the same position was also positive,and positive intensity of the PCNA expression was consistent with severity of local lesions. Conclusion There are regeneration,proliferation and differentiation of injured renal tubular epithelial cells at early stage of the EHF patients with acute renal failure. These changes of the renal tubular epithelial cells are of great significance during restoration of injured kidneys.

关 键 词：流行性出血热 肾脏 增殖细胞核抗原 波形丝蛋白 

分 类 号：R512.8[医药卫生—内科学]