务川黑牛微卫星座位遗传多样性分析  

The Analysis of Genetic Diversity of Microsatellite Loci in Wuchuan Black Cattle

作　　者：吴芸[1] 蒋自立[1] 田雄 马培江 

机构地区：[1]遵义师范学院生物系,贵州遵义563000 [2]务川仡佬族苗族自治县饲草饲料站,贵州务川564300 [3]务川仡佬族苗族自治县柏村镇畜牧兽医站,贵州务川564300

出　　处：《河南农业科学》2012年第12期141-145,共5页Journal of Henan Agricultural Sciences

基　　金：遵义市科技项目(遵市科合社字2010-09)

摘　　要：以贵州地方牛优良品种务川黑牛为研究对象,选取分布于其7条染色体上的ETH10、ILSTS033、CSSM66、BM2113、ETH225、BM1818和CSRM60共7对微卫星引物进行遗传多样性分析。结果共检测到21个等位基因,每个微卫星座位等位基因数为2～5个,7个微卫星座位均未达到Hardy-Weinberg平衡。7个位点的平均等位基因数、平均有效等位基因数、平均期望杂合度、平均多态信息含量分别为3.000 0±1.154 7、2.377 3±0.585 4、0.558 4±0.101 6、0.475 9±0.125 1,说明务川黑牛具有良好的遗传多样性。为务川黑牛种质资源保存和改良提供了试验基础。Wuchuan black cattle is a good local breed in Guizhou province. In this study, the genetic diversity of Wuchuan black cattle was analyzed by 7 selected pairs of microsatellite loci which distribute on seven different cattle chromosomes, including ETH10, ILSTS033, CSSM66, BM2113,ETH225,BM1818 and CSRM60. The results showed that 21 alleles were found,the allele numbers of each lOCUS changes from 2 to 5 and all of these microsatellite loci didn＇t reached the Hardy-Weinberg equilibrium. The average number of alleles, average effective number of alleles, average expected heterozygosity and average polymorphism information content were 3. 000 0±1. 154 7,2. 377 3±0. 585 4,0. 558 4±0. 101 6 and 0. 475 9±0. 125 1,respectively. All the data represent us a good genetic diversity of Wuchuan black cattle. To provide an experimental basis for germplasm consenation and improvement of Wuchuan black cattle.

关 键 词：务川黑牛 微卫星 遗传多样性 遗传变异 

分 类 号：S823.8[农业科学—畜牧学]