人正常胃肠道、生殖道粘膜中MUC5AC核粘蛋白及其mRNA定位的研究  

MUC5AC GENE EXPRESSION IN THE TISSUES OF THE NORMAL GASTROINTESTINAL AND GENITAL TRACTS

作　　者：汪荣泉[1] 房殿春[1] 刘为纹[1] 晋华源[1] 

机构地区：[1]第三军医大学西南医院消化科,重庆400038

出　　处：《解剖学杂志》2000年第3期216-219,共4页Chinese Journal of Anatomy

基　　金：全军"九五"医药卫生科研基金(No.96M091)

摘　　要：目的:揭示MUC5AC基因在人胃肠道和生殖道粘膜组织中的表达异质性及其特点,为进一步研究其与疾病关系提供正常的形态学基础。方法:应用免疫组织化学和原位杂交方法。结果:MUC5AC基因编码的核粘蛋白及其mRNA主要分布于人正常胃粘膜表面1/3,包括粘膜的上皮层和胃腺的中上部,呈细颗粒状,位于阳性细胞的胞浆内,核周明显,胃窦、胃底的表达无区别。十二指肠、空肠、结肠及宫颈组织内无表达,胆囊粘膜内灶状分布的阳性染色细胞。结论:MUC5AC基因主要在胃和胆囊组织内表达,而小肠、结肠、宫颈无表达,提示MUC5AC基因表达存在组织异质性。Objective: To elucidate the distributive heterogeneity of MUC5AC gene in human normal gastrointestinal and genital tracts and its characteristics.' Methods : The expression of MUC5AC gene were detected by immunohistochemical SP method and in situ hybridization. Results- The MUC5AC apomucin and its mRNA were mainly distributed in the 1/3 superjacent gastric mu-cosa, including the superficial epithelium and gastric glandulae. The apomucin and its mRNA was situated in the cytoplasm, especially around the nuclei, most of the positive staining features were fine granular. No staining difference was found between fundus and antrum tissues. There was no positive staining in the tissues of duodenum, jejunum, colon and cervix uteri. Clustered positively staining cell was found in the mucosa of gallbladder. Conclusion; The expression of MUC5AC gene is mainly in the tissues of stomach and gallbladder, not in the duodenum, colon and cervix uteri. This facts indicate that MUC5AC expression heterogeneous and organ-specific.

关 键 词：胃肠道 生殖道 粘膜 MUC5AC 核粘蛋白 MRNA 

分 类 号：R346[医药卫生—基础医学]