水稻光氧化基因LPO1(t)的初步定位  

Mapping of a leaf photo-oxidation gene LPO1(t)in rice

作　　者：赖东[1] 夏士健[2] 吕川根[2] 魏晓东[2] 刘少奎[1] 张斌[1] 廖慧敏[1] 颜文飞[1] 宗寿余[2] 张启军[2] 

机构地区：[1]南京农业大学农学院,江苏南京210095 [2]江苏省农业科学院粮食作物研究所,江苏省优质水稻工程技术研究中心,江苏南京210014

出　　处：《江苏农业学报》2012年第6期1212-1217,共6页Jiangsu Journal of Agricultural Sciences

基　　金：国家转基因专项(2011ZX08001-004)

摘　　要：在选育的籼型两用不育系812S中分化出1个农艺性状基本一致的光氧化变异株系812HS,秧苗期叶色正常,但分蘖盛期～拔节期,在低温强光照射下,叶尖发生光氧化而变黄。生理分析结果表明,812HS叶片的PSⅡ反应中心活力和PSⅡ电子传递活力低于野生型,而剩余光能高于野生型,光能利用效率低于野生型,说明812HS的性状是由已合成的叶绿素受光氧化伤害造成的。以812HS/090028 F1和F2群体为材料进行遗传分析,结果表明,该光氧化性状受1对新的显性主基因LPO1(t)控制。采用BSA法,用微卫星SSR标记将LPO1(t)基因初步定位于第4染色体上的分子标记RM307与RM401之间,LPO1(t)与2个标记间的遗传距离分别为4.3 cM和4.5 cM。LPO1(t)是水稻中一个与其他叶片光氧化相关基因不同的新基因。In the present experiment,a photo-oxidation mutant,designated as 812HS,derived from a segregation population of an indica photo-thermo-sensitive male sterile line 812S was found.812HS showed the same agronomic traits as 812S in the seedling stage,however,the leaves of 812HS turned yellow from the top by photo-oxidation when exposed to strong sunlight at a low temperature at tillering and jointing.Physiological analysis showed that the activities of PSⅡreaction center（RC） and PSⅡ electron transport of the mutant were significantly reduced in comparison with its wild-type,however,remaining energy of PSⅡ reaction center was increased,and the light use efficiency was reduced.The leaves etiolating of the mutant was arisen by photooxidized chlorophyll.Genetic analysis of F1 and F2 populations of 812HS/090028（an indica line from IRRI） showed that photo-oxidation was controlled by a new single dominant gene,LPO1（t）.Using BSA（bulk segregant analysis） and LPO1（t） was mapped primarily between RM307 and RM401,with genetic distances of 4.3 cM and 4.5 cM,respectively,on Chromosome 4.LPO1（t） gene is a new gene distinguished from all other genes related to leaf photo-oxidation in rice.

关 键 词：水稻 光氧化 简单重复序列标记 基因 定位 

分 类 号：S511.01[农业科学—作物学]