罗汉果组培苗脱毒方法研究  

Study on virus-free of Siraitia grosvenorii in tissue culture seedling

作　　者：吴群英[1] 李伯林[2] 李景云[3] 

机构地区：[1]桂林医学院生物技术学院,广西桂林541004 [2]广西师范大学生命科学学院,广西桂林541004 [3]广西中医药大学基础医学院,广西南宁530001

出　　处：《广东农业科学》2012年第24期18-20,共3页Guangdong Agricultural Sciences

基　　金：广西科技攻关项目(桂科攻0630002-3F);广西教育厅科研项目(桂教教科研字[2000]392号)

摘　　要：采用热处理结合茎尖技术对罗汉果组培苗进行脱毒培养，并用间接酶联免疫法和电镜观察法对脱毒效果进行检测．结果表明．组培苗经38．5℃热处理10d后，剥取0．2～0．5mm微茎尖培养或切取1．0～1．5mm茎尖进行二次茎尖脱毒培养，茎尖苗的脱毒率均为100％，建立了两种有效的罗汉果组培苗脱毒方法。Siraitia grosvenorii in vitro were used to eliminate Luohanguo mosaic virus （WMV-2-Luo）, by combing tissue culture of shoot-tip with heat therapy, using indirect ELISA method and electron microscope for virus detection. The results shown that heat treatment by 38.5℃ for 10 days of Siraitia grosvenorii, the detoxication rate of 0.2-0.5 mm micro-stem tip or 1.0-1.5 mm twice shoot-tip culture, was 100% free from WMV-2-Luo, which set up two kinds of effective techniques of virus-free of Siraitia grosvenorii in tissue culture seedling.

关 键 词：罗汉果花叶病毒 茎尖培养 酶联免疫法 脱毒 

分 类 号：R282.21[医药卫生—中药学]