基于SSR标记的广东省甘蔗黑穗病菌遗传多样性研究  

Genetic diversity analysis of sugarcane smut fungus in Guangdong providence revealed by SSR markers

作　　者：陈健文[1] 冯淑杰[2] 钟玫[2] 刘睿[1] 沈万宽[1] 陈仲华[1] 杨湛端[1] 徐苑娴[1] 邓海华[1] 

机构地区：[1]广州甘蔗糖业研究所/广东省甘蔗改良与生物炼制重点实验室,广东广州510316 [2]华南农业大学园艺学院,广东广州510642

出　　处：《广东农业科学》2012年第24期154-157,共4页Guangdong Agricultural Sciences

基　　金：广东省科技计划项目(粤科规划字[2009]198号);国家现代农业产业体系建设(CARS-20)

摘　　要：利用SSR标记分析了30个来自广东省甘蔗主产区的甘蔗黑穗病菌菌株的遗传多样性。用19对带型较稳定且条带清晰的引物从供试菌株群体中检测到了39个多态性位点,平均每个引物得到2.05条多态性带。UPGMA聚类分析表明,在约0.74的相似系数水平,SSR标记把30个菌株划分为5个类群,类群1有22个菌株,包含了绝大部分来自粤西和粤北的菌株及全部的广州市郊菌株;在约0.8的相似系数水平,类群1可以进一步分为2个亚群体,亚群体1包含了90%的粤北菌株,亚群体2则包含了50%的粤西菌株。研究结果表明:该菌的遗传多样性与菌株的地理来源呈显著相关,而与品种间的相关性不大。The genetie diversity of 30 sugarcane smut strains from the main sugarcane growing area of Guangdong province was studied by simple sequence repeats （SSR） markers. 16 pairs of SSR primers, showing stable amplified band pattern and clear bands, were selected to analysis these strains. A total of 39 polymorphic loci were obtained and 2.05 polymorphic loci per SSR primer were detected. Based on SSR marker data at the similarity coefficient level of about 0.74, these 30 strains were divided into 5 groups by UPGMA clustering analysis. The first group consisted of 22 strains and could be further divided into 2 subgroups at the similarity coefficient level of about 0.8. The subgroup one included 90 percent strains, which originated from the north of Guangdong, while the subgroup two contained 50 percent strains, which collected from the west of Guangdong. The results showed that the genetic diversity of sugarcane smut was associated with geographical origin, but not related to the host.

关 键 词：甘蔗黑粉菌 遗传多样性 SSR标记 

分 类 号：Q341[生物学—遗传学]