PTD-Cry3Aa原核融合表达产物对甘薯小象甲的活性研究  

A Study on the Activity of the Prokaryotic Expression Products of PTDCry3Aa Fusion Gene to Sweet Potato Weevil,Cylas formicarius

作　　者：邱道寿[1] 刘晓津[1] 

机构地区：[1]广东省农业科学院作物研究所/广东省农作物遗传改良重点实验室,广东广州510640

出　　处：《生物灾害科学》2012年第4期347-351,共5页Biological Disaster Science

基　　金：广东省自然科学基金项目(06025389)

摘　　要：为探讨TAT PTD对苏云金杆菌鞘翅目特效基因CryAa是否具有协同作用,本试验在实现了融合基因TAT PTD-Cry3Aa大肠杆菌原核表达的基础上,以甘薯小象甲为生测对象测定了原核表达产物PTD-Cry3Aa与Cry3Aa的毒力。结果表明,用大肠杆菌表达的Cry3A和PTD-Cry3Aa包涵体初提物均对甘薯小象甲成虫和幼虫有杀虫活性,且表现出一定毒力增效作用,对成虫的增效倍数为2.01倍,对幼虫的增效作用更加明显,达到3.19倍。这一研究结果对于甘薯小象甲的生物防治研究以及转基因抗虫甘薯的研究具有重要的指导作用。In order to study the synergism of the TAT PTD（the protein transduction domain in HIV1 transactivator of transcription protein） to the Cry3Aa from Bacillus thuringiensis,the fusion gene PTDCry3Aa and gene Cry3Aa were expressed in E.coli.cells.The toxicity of prokaryotic expression products from PTDCry3Aa and Cry3Aa was tested in sweet potato weevil,Cylas formicarius.The bioassay results showed that the primary extracts of PTDCry3Aa and Cry3Aa inclusion bodies exhibited activities against the adults and larvae of sweet potato weevil.The results also indicated there was a synergism of PTD to Cry3Aa both on the adults and the larvae.The synergistic rates were 2.01 folds for the adults while 3.19 folds for the larvae.The study results would play an important role in biological control and transgenic sweet potato against sweet potato weevil.

关 键 词：Cry3Aa 蛋白转导结构域(PTD) 甘薯小象甲 

分 类 号：S435.315.1[农业科学—农业昆虫与害虫防治]