大白菜×小白菜双单倍体永久作图群体的创建  

Establishing Doubled Haploid Population for Genetic Mapping in Chinese Cabbage × Pakchoi

作　　者：章云[1] 冯辉[1] 

机构地区：[1]沈阳农业大学园艺学院,沈阳110161

出　　处：《沈阳农业大学学报》2012年第4期425-429,共5页Journal of Shenyang Agricultural University

基　　金：国家自然科学基金项目(31071792)

摘　　要：对芸薹属芸薹种不同亚种间大白菜福田50双单倍体纯系FT50和小白菜双单倍体纯系HG01杂交的F1植株进行小孢子培养,在培养基中加入不同浓度的6-BA和NAA,研究其对胚状体诱导和植株再生的影响。结果表明:最适的小孢子诱导培养基为NLN-13+0.05mg.L-16-BA+0.05mg.L-1NAA,诱导率可达14.13胚.蕾-1。最适的生根培养基为MS+0.10mg.L-1NAA+3%蔗糖+0.55%琼脂,生根率为100.00%,再生植株驯化后移栽到到花盆中,在日光温室培育,采取形态学和流式细胞仪结合方法鉴定再生植株倍性,自然加倍率为56.67%。人工蕾期自交得到一个具有242个双单倍体的作图群体。Microspore culture was carried out in hybrid plants between a Chinese cabbage DH line FT50 and a Pakchoi DH line HG01, which are belong to different subspecies of Brassica rapa, respectively. The medium was added with 6-BA and NAA to study its influence on embryogenesis and plant regeneration. The results showed that the suitable medium for microspore embryogenesis was NLN-13＋0.05 mg·L^-1 6-BA＋ 0.05mg·L^-1 NAA. The embryogenesis frequency was 14.13 embryos per bud. The suitable medium for rooting was MS＋0.1mg·L^-1 NAA ＋ 3% sugar ＋ 0.55% agar and. The root inducing rate reached to 100.00%. The regenerated plants were transplanted to plots in the greenhouse. Ploidy evaluation was identified by DNA flow eytometry and the method of morphological characteristics, the doubling efficiency was 56.67%. A DH population with 242 lines was obtained.

关 键 词：大白菜 小白菜 小孢子培养 双单倍体 作图群体 

分 类 号：S634.1[农业科学—蔬菜学]