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作 者:王利[1] 崔娜曲[1,2] 范海延[1] 苗青[1] 曲波[1] 李天来[2]
机构地区:[1]沈阳农业大学,生物科学技术学院,沈阳110161 [2]沈阳农业大学,设施园艺省部共建教育部重点实验室,沈阳110161
出 处:《沈阳农业大学学报》2012年第4期482-485,共4页Journal of Shenyang Agricultural University
基 金:国家科技支撑项目(2011BAD12B03);公益性行业(农业)科研专项项目(201103027)
摘 要:选用野生型番茄克梅留斯基(Solanum chmielewskii)和普通栽培型番茄Micro-Tom(Solanum lycopersicum)为试验材料,比较两种不同类型番茄花后不同生长阶段叶片中碳水化合物代谢的动态变化,明确其在开花后叶片碳水化合物代谢的规律,为以Micro-Tom为试材的糖代谢研究及野生型番茄资源的利用服务。结果表明:在番茄开花后25d,叶片中果糖、葡萄糖含量Micro-Tom明显高于克梅留斯基,分别高出247%和290%;花后25,35d,45d Micro-Tom叶片中蔗糖含量高于克梅留斯基,分别高出111%、96%和169%;而克梅留斯基在花后15d,叶片中淀粉含量明显高于Micro-Tom,为142%。转化酶(酸性转化酶和中性转化酶)活性在花后25d Micro-Tom叶片明显高于克梅留斯基,分别高348%和138%;而整个果实发育过程中,Micro-Tom叶片的蔗糖合成酶活性都高于克梅留斯基,蔗糖磷酸合成酶活性则是克梅留斯基较高。Two kinds of tomato Solanum chmielewskii and Micro-Tom were used to studcy the dynamic changes of carbohydrate in their leaves at different growth stages and define the carbohydrate metabolism after anthesis, providing a referancc for the study of sugar metabolism of Micro-Tom and the utilization of wild tomato resources. The main results showed that the contents of fructose and glucose in the leaves of Micro-Tom were significantly 247% and 290% higher than those of Solanum chmielewskii at 25 days after anthesis and the sucrose contents in the leaves of Micro-Tom were 111%, 96% and 169% higher than those of SoIanum chmielewskii at 25 days, 35 days and 45 days after anthesis, respectively; the content of starch in the leaves of Solanum chmielewskii was 142% higher than that of Micro-Tom. Activities of invertases including acid invertase and neutral invertase in Micro-Tom were 348% and 138% higher than those in Solanum chmielewskii at 25 days alter anthesis; sucrose synthase in Micro-Tom was always higher than that in Solanum chmielewskii at the same stage, however, sucrose phosphate synthase in Solanum chmielewskii was always higher during the whole development of fruits.
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