电化学发光法检测单纯疱疹病毒2 IgG抗体应用评估  

Application evaluation of electro-chemiluminescence assay for the detection of anti-herpes simplex virus type 2 IgG antibody

作　　者：陈戴红[1] 毛会军[1] 邱建萍[1] 吴文娟[1] 

机构地区：[1]上海市公共卫生临床中心医学检验科,上海201508

出　　处：《检验医学》2012年第11期887-889,共3页Laboratory Medicine

摘　　要：目的对电化学发光法(ECLIA)和酶联免疫吸附试验(ELISA)检测单纯疱疹病毒2(HSV-2)IgG抗体结果进行比较分析,评价ECLIA的性能及临床应用价值。方法应用罗氏诊断公司ECLIA与爱尔兰Trinity公司ELISA试剂分别检测400份血清标本中的HSV-2特异性IgG抗体,检测结果不一致的标本用欧蒙生物技术有限公司的抗HSV-2 IgG抗体检测试剂进行复核。结果 ELISA和ECLIA检测阳性率分别为39.0%、37.8%;以ELISA为参照,ECLIA假阴性率为1.3%,假阳性率为0.8%,相对敏感性为96.8%,相对特异性为98.8%,二者的符合率为98.0%。使用欧蒙生物技术有限公司试剂对13份检测结果不一致(包括2个不确定结果)的标本进行了复核,其中6份标本结果支持ECLIA,5份标本结果支持ELISA。结论 ECLIA和ELISA检测HSV-2 IgG抗体结果符合情况良好,罗氏"HSV-2 IgG抗体检测试剂(ECLIA)"是一种快速、准确、敏感性和特异性高的试剂,可以应用于临床。Objective To compare the results of anti-herpes simplex virus type 2（HSV-2）IgG antibody detected by electro-chemiluminescence assay（ECLIA）and enzyme-linked immunosorbent assay（ELISA）,and evaluate the performance and application significance of ECLIA.Methods Anti-HSV-2 specific IgG antibody of 400 serum samples were detected by ECLIA（Roche）and ELISA（Trinity,Ireland）.The samples with inconsistent results were detected by anti-HSV-2 IgG antibody detection kit of Euroimmun Biotechnology Co.,Ltd..Results The positive rates were 39.0% by ELISA and 37.8% by ECLIA respectively.Compared with ELISA,the false negative rate of ECLIA was 1.3%,and the false positive rate was 0.8%.The relative sensitivity and relative specificity were 96.8% and 98.8%.The coincidence rate of the 2 methods was 98.0%.The 13 samples with inconsistent results（including 2 uncertain results）were detected by anti-HSV-2 IgG antibody detection kit of Euroimmun Biotechnology Co.,Ltd..The results indicated that 6 samples were consistent with ECLIA,and 5 samples were consistent with ELISA.Conclusions ECLIA and ELISA results had good coincidence,and Roche ECLIA can be used as a fast,accurate,sensitive,high-specific reagent for clinical application in the determination of anti-HSV-2 IgG antibody.

关 键 词：单纯疱疹病毒2 IGG抗体 电化学发光法 酶联免疫吸附试验 

分 类 号：R446.61[医药卫生—诊断学]