紫外分光光度法测定细基丸茎和叶总异黄酮的含量  

Ultraviolet Spectrophotometric Determination of Total Isoflavones in the Stems and Leaves of Polyalthia cerasoides

作　　者：刘冰晶[1] 曾靖[1] 李超[1] 江智伟[1] 宋鑫明[2] 

机构地区：[1]赣南医学院药学院江西省脑血管药理重点实验室,江西赣州341000 [2]海南师范大学化学与化工学院"省部共建"热带药用植物教育部重点实验室,海南海口571158

出　　处：《赣南医学院学报》2012年第6期809-811,共3页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：海南省自然科学基金(20604);赣南医学院校级项目(YB201116)

摘　　要：目的:测定细基丸茎、叶中总异黄酮的含量。方法:以染料木素为对照品,产生反应,采用紫外-可见分光光度法在266 nm波长处测定样品的吸光度。结果:染料木素对照品在0.20～1.60μg/mL范围内呈良好线性关系,回归方程为C=0.003 9 A,R=0.999 9。细基丸茎和叶中的总异黄酮含量分别为0.120 2%和0.231 5%。结论:本法操作简便,结果可靠,重复性好,可用作细基丸中总异黄酮的含量测定。Objective： To determine the content of the isoflavones in the stem and leaves of Polyalthia cerasoides.Methods： Genistein as the standard,used the ultraviolet spectrophotometric method to determine the content of the isoflavones in the stems and leaves of P.cerasoides.Results： The calibration curve was linear（R = 0.999 9） in the range of 0.000 5 ～ 0.004 5 μg / mL,and the curve was C = 0.003 9 A,The content of stems was about 0.120 2% and content of leaves was about0.231 5%.Conclusion： The spectrophotometry is suitable for the determination of isoflavones from the stems and leaves of P.cerasoides.This method was operated conveniently,the result was stable,and has good repeatability.

关 键 词：细基丸 总异黄酮 紫外分光光度法 

分 类 号：O657.32[理学—分析化学]