偶氮胭脂红B共振散射光谱法测定硫酸庆大霉素  

Determination of Gentamycin Sulfate with Azocarmine B by Resonance Light Scattering Spectrometry

作　　者：唐宁莉[1] 凌悦菲[1] 蒙华毅[1] 李曾奎[1] 

机构地区：[1]桂林理工大学化学与生物工程学院,广西桂林541004

出　　处：《桂林理工大学学报》2012年第4期610-612,共3页Journal of Guilin University of Technology

基　　金：国家自然科学基金项目(21165008)

摘　　要：在pH 2.8的Britton-Robinson缓冲溶液中,偶氮胭脂红B与硫酸庆大霉素相互作用形成缔合物,使体系的共振散射信号(RLS)增强,最大散射峰位于285 nm处,且增强的RLS强度与硫酸庆大霉素浓度成正比,据此建立了一种测定硫酸庆大霉素含量的新方法:其线性范围0.02～3.5μg/mL,检出限0.012μg/mL。方法用于硫酸庆大霉素注射液中硫酸庆大霉素含量的测定,结果满意。In Britton-Robinson buffer solution of pH 2.8,when azocarmine B reacts with gentamycin sulfate（GEN） to form association complex,the resonance light scattering signal（RLS） of system can be enhanced.The maximum scattering peak is 285 nm and the enhanced RLS intensity is proportional to the concentration of GEN.Based on that,a new method for the determination of GEN is developed.The linear range is 0.02 ～ 3.5 μg/mL and the detection limit is 0.012 μg/mL.The application of determination of GEN in injection samples provides satisfactory result.

关 键 词：硫酸庆大霉素 偶氮胭脂红B 共振散射光谱法 

分 类 号：O657.32[理学—分析化学]