复方中药多糖对鸡新城疫疫苗免疫效果的影响  

Effect of Compound Polysaccharide on Immune Efficacy of Newcastle Disease Vaccine in Chicken

作　　者：乔海博[1] 谷新利[1] 朱晓庆[1] 

机构地区：[1]石河子大学动物科技学院,石河子832003

出　　处：《石河子大学学报（自然科学版）》2012年第6期700-703,共4页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金项目(30960291)

摘　　要：为探究前期研究效果显著的最佳纯度多糖对人工免疫新城疫疫苗的雏鸡抗体滴度和抗氧化能力的影响,将350只雏鸡随机分为7组(空白对照组,免疫对照组,盐酸左旋咪唑组,黄芪多糖组,最佳纯度复方中药多糖高剂量、中剂量、低剂量组),除空白组外,其余雏鸡在7日龄用新城疫活疫苗La sota株免疫,在27日龄用新城疫活疫苗CS2株进行二次免疫,8至14日龄给药,各组雏鸡在15、22、29、36、43和50日龄采集血样,测定鸡新城疫疫苗抗体滴度和超氧化物歧化酶浓度。结果显示:最佳纯度复方中药多糖中低剂量均可极显著提高新城疫抗体滴度(P<0.05),其中以中剂量效果最好;黄芪多糖和最佳纯度复方中药多糖高中低剂量均可显著提高鸡超氧化物歧化酶含量(P<0.05),其中以最佳纯度复方中药多糖中剂量效果最好(P<0.01)。To explore the effect of optimum purity polysaccharide,which is proved to have immune effect in previous study,on the Newcastle disease（ND） antibody titer and activity of Superoxide Dismutase（SOD）,350 chicks were randomly divided into 7 groups（blank control,immunization control,levamisole hydrochloride,astragalus polysaccharide,high,middle and low dosage of optimum purity polysaccharide）.ND vaccine was immunized in every group except blank control at 7 and 27 days old.Blood samples of every group were collected on 15,22,29,36,43 and 50 day to determine antibody titer and SOD activity after given mediation in 8～14days.The results showed that the medium and low dosages of optimum purity polysaccharide improved the antibody titer of immunized chicks significantly（P0.05）,and the medium dosage got the best effect;astragalus polysaccharide and three dosages of optimum purity polysaccharide were obviously stronger than the others on SOD（P0.05）,especially for the medium dosage（P0.01）.

关 键 词：复方中药多糖 新城疫疫苗 抗体滴度 超氧化物歧化酶 鸡 

分 类 号：S853.7[农业科学—临床兽医学] S858.31[农业科学—兽医学]