洋葱总黄酮的大孔吸附树脂纯化工艺  

Purification of Onion Total Flavonoids by Macroporous Adsorption Resin

作　　者：王国军[1] 唐辉[1] 王幻[1] 王鲁石[1] 

机构地区：[1]石河子大学药学院/新疆特种植物药资源重点实验室,石河子832002

出　　处：《石河子大学学报（自然科学版）》2012年第6期731-734,共4页Journal of Shihezi University(Natural Science)

基　　金：石河子大学科技成果转化引导资金专项(KJKFYZH2009-CGZH02)

摘　　要：为研究大孔吸附树脂分离纯化洋葱总黄酮的工艺条件及参数。以洋葱总黄酮吸附率与解析率为指标,考察了2种不同型号的大孔树脂对洋葱总黄酮的吸附和解吸附能力,确定AB-8为最佳树脂,并对AB-8大孔树脂的纯化条件进行优化。结果显示,最佳工艺条件为pH=4、1～2mg/mL的浓度上样,上样流速为1.5BV/h,样品上样量为3mL/g树脂;用60%乙醇洗脱。经AB-8树脂处理后的总黄酮纯度达69.8%,收率为86.8%。由此可知,AB-8树脂对洋葱总黄酮的吸附量大,解吸附容易,可以应用于洋葱总黄酮的分离纯化。The process parameters of the separation and purification of onion total flavonoids with macroporous adsorption resins were studied.Two kinds of macroporous adsorption resins were studied on the static capacity of adsorption and desorption,and the better resin was AB-8.The optimum adsorption and desorption parameters of purification onion total flavonoids with resin AB-8 were as follows：pH=4,adsorption concentration of total flavonoids is 1～2 mg/mL,flow rate 1.5BV/h,quantity of the sample is 3mL/g,elution solvent 60% alcohol for desorption.The purification of onion total flavonoids with macroporous adsorption resins AB-8 was about 69.8%,and percent yield was 86.8%.The results show that resin AB-8 has good ability of purification of onion total flavonoids,and it could be used to separate and purify onion total flavonoids.

关 键 词：大孔吸附树脂 洋葱总黄酮 分离纯化 吸附 解吸附 

分 类 号：TQ461[化学工程—制药化工]